Quantitation of TNP-470 and its metabolites in human plasma: sample handling, assay performance and stability

被引：11

作者：

Ong, VS ^{[1
]}

Stamm, GE ^{[1
]}

Menacherry, S ^{[1
]}

Chu, SY ^{[1
]}

机构：

[1] Abbott Labs, Drug Metab, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 710卷 / 1-2期

关键词：

TNP-470;

D O I：

10.1016/S0378-4347(98)00111-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A selective and sensitive assay for the determination of TNP-470 and two of its metabolites, AGM-1883 and M-II, in human plasma was developed. The assay involved liquid-liquid extraction followed by analysis using high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry. Because TNP-470 is most stable in a pH of 4-5, an acidification procedure was utilized to prevent degradation of TNP-470 during sample collection which involved acidifying the whole blood sample collected with 5 mg of citric acid per mi of blood. Liquid-liquid extraction using an organic solvent mixture was chosen over solid-phase extraction to minimize the degradation of TNP-470 during solvent evaporation. (C) 1998 Elsevier Science B.V. All rights reserved.

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页码：173 / 182

页数：10

相关论文

共 11 条

[1] Pharmacokinetics and metabolism of O-(chloroacetyl-carbamoyl) fumagillol (TNP-470, AGM-1470) in rhesus monkeys [J].

CrettonScott, E ;

Placidi, L ;

McClure, H ;

Anderson, DC ;

Sommadossi, JP .

CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1996, 38 (02) :117-122

[2] SYNTHETIC ANALOGS OF FUMAGILLIN THAT INHIBIT ANGIOGENESIS AND SUPPRESS TUMOR-GROWTH [J].

INGBER, D ;

FUJITA, T ;

KISHIMOTO, S ;

SUDO, K ;

KANAMARU, T ;

BREM, H ;

FOLKMAN, J .

NATURE, 1990, 348 (6301) :555-557

[3] Determination of O-(chloroacetylcarbamoyl)fumagillol (TNP-470;AGM-1470) and two metabolites in plasma by high-performance liquid chromatography mass spectrometry with atmospheric pressure chemical ionization [J].

Moore, JD ;

Sommadossi, JP .

JOURNAL OF MASS SPECTROMETRY, 1995, 30 (12) :1707-1715

[4] TREATMENT OF MURINE HEMANGIOENDOTHELIOMAS WITH THE ANGIOGENESIS INHIBITOR AGM-1470 [J].

OREILLY, MS ;

BREM, H ;

FOLKMAN, J .

JOURNAL OF PEDIATRIC SURGERY, 1995, 30 (02) :325-330

[5] Antiangiogenic therapy of transgenic mice impairs de novo tumor growth [J].

Parangi, S ;

OReilly, M ;

Christofori, G ;

Holmgren, L ;

Grosfeld, J ;

Folkman, J ;

Hanahan, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (05) :2002-2007

[6]

SAVILLE MW, 1993, CLIN RES, V41, pA161

[7] THE INFLUENCE OF ANGIOGENESIS INHIBITOR AGM-1470 ON IMMUNE-SYSTEM STATUS AND TUMOR-GROWTH IN-VITRO [J].

SCHOOF, DD ;

OBANDO, JA ;

CUSACK, JC ;

GOEDEGEBUURE, PS ;

BREM, H ;

EBERLEIN, TJ .

INTERNATIONAL JOURNAL OF CANCER, 1993, 55 (04) :630-635

[8]

TANAKA T, 1995, CANCER RES, V55, P836

[9]

TEICHER BA, 1995, ONCOL RES, V7, P237

[10] POTENTIATION OF CYTOTOXIC CANCER THERAPIES BY TNP-470 ALONE AND WITH OTHER ANTI-ANGIOGENIC AGENTS [J].

TEICHER, BA ;

HOLDEN, SA ;

ARA, G ;

SOTOMAYOR, EA ;

HUANG, ZD ;

CHEN, YN ;

BREM, H .

INTERNATIONAL JOURNAL OF CANCER, 1994, 57 (06) :920-925