Determination of the change of flavonoid components as the defence materials of Citrus unshiu Marc. fruit peel against Penicillium digitatum by liquid chromatography coupled with tandem mass spectrometry

被引:70
作者
Kim, Hae Gyeong [1 ,2 ]
Kim, Gon-Sup [2 ,3 ]
Lee, Jung Han [1 ,2 ]
Park, Semin [1 ,2 ]
Jeong, Won Young [1 ,2 ]
Kim, Yun-Hi [1 ,2 ]
Kim, Jae Hoon [4 ]
Kim, Soo Taek [5 ,6 ]
Cho, Young Ah [1 ,2 ]
Lee, Won Sup [7 ,8 ]
Lee, Soo Jung [9 ]
Jin, Jong Sung [10 ]
Shin, Sung Chul [1 ,2 ]
机构
[1] Gyeongsang Natl Univ, Dept Chem, Jinju 660701, South Korea
[2] Gyeongsang Natl Univ, Life Sci Res Inst, Jinju 660701, South Korea
[3] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, South Korea
[4] Jeju Natl Univ, Coll Appl Life Sci, Citrus Genet Resources Bank, Fac Biotechnol, Cheju 690756, South Korea
[5] Gyeongsang Natl Univ, Res Inst Nat Sci, Jinju 660701, South Korea
[6] Gyeongsang Natl Univ, Dept Informat & Stat, Jinju 660701, South Korea
[7] Gyeongsang Natl Univ, Gyeongnam Reg Canc Ctr, Jinju 660702, South Korea
[8] Gyeongsang Natl Univ, Inst Hlth Sci, Dept Internal Med, Jinju 660702, South Korea
[9] Gyeongsang Natl Univ, Inst Agr & Life Sci, Dept Food & Nutr, Jinju 660701, South Korea
[10] Korea Basic Sci Inst Busan Ctr, Div High Technol Mat Res, Pusan 618230, South Korea
关键词
Citrus unshiu Marc; Flavanones; Flavones; Defence materials; High-performance liquid chromatography; Tandem mass spectrometry; POLYMETHOXYLATED FLAVONES; PHYTOPHTHORA-CITROPHTHORA; RESISTANCE; MECHANISM; POLYMETHOXYFLAVONES; DIFFERENTIATION; FLAVANONES; EXTRACT;
D O I
10.1016/j.foodchem.2011.02.075
中图分类号
O69 [应用化学];
学科分类号
070301 [无机化学];
摘要
A healthy fruit peel of Citrus unshiu Marc. and one infected by Penicillium digitatum were analysed for flavonoids via high-performance liquid chromatography coupled with tandem mass spectrometry (IPLC-MS/MS) in the positive mode with selected ion monitoring (SIM). Among 16 flavonoid components characterised in C. unshiu Marc., four flavanones and nine flavones were identified for the first time. The identified compounds were quantified by HPLC-UV. To investigate the function of the flavonoids as defence materials, the flavonoid content change of the fruit peel inoculated with P. digitatum was monitored by HPLC. The flavonoid concentration in the infected fruit peel decreased initially after the infection and then gradually increased before finally progressively decreasing. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved,
引用
收藏
页码:49 / 54
页数:6
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