The use of wavelets for signal denoising in capillary electrophoresis

The discrete wavelet transform was applied to denoise electropherograms in capillary electrophoresis (CE). The use of the Haar wavelet and translation invariant denoising were found to be very efficient for this purpose. An important improvement was obtained, as compared with Savitzky-Golay and Fourier, which are the most commonly used techniques for denoising in the instrumentation software packages. A better removal of the noise and, especially, a better preservation of the shapes of very sharp peaks was achieved. Removal of the baseline variations was also investigated.