Nitrogen enrichment causes minimal changes in arbuscular mycorrhizal colonization but shifts community composition-evidence from rDNA data

被引:78
作者
Jumpponen, A [1 ]
Trowbridge, J [1 ]
Mandyam, K [1 ]
Johnson, L [1 ]
机构
[1] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA
基金
美国国家科学基金会;
关键词
arbuscular mycorrhiza; nitrogen deposition; Ribosomal DNA; tallgrass prairie;
D O I
10.1007/s00374-005-0845-8
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Intracellular arbuscular mycorrhizal ( AM) colonization was compared between nitrogen (NH4NO3) fertilized (10 g N m(-2)) and nonfertilized tallgrass prairie plots. In the microscopic analyses of host roots, only intracellular coils showed an increasing trend as a result of N fertilization, whereas intracellular colonization by arbuscules, hyphae, or vesicles did not differ between the N treatments. Clone libraries established from pooled PCR products of AM fungi contained exclusively species of Glomus; no other genera were detected indicating that Glomus spp. dominated the host roots. Comparisons between observed and random topologies indicated that cloned sequence placement covaried with N treatment: unique clades within Glomus originated exclusively from N-fertilized or nonfertilized treatments. We conclude that the communities of dominant and most commonly occurring AM fungi changed in response to N amendment, although the root colonization showed minimal or no response.
引用
收藏
页码:217 / 224
页数:8
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