High and low UV-dose responses in SOS-induction of the precise excision of transposons Tn1, Tn5 and Tn10 in Escherichia coli

被引：16

作者：

Aleshkin, GI ^{[1
]}

Kadzhaev, KV ^{[1
]}

Markov, AP ^{[1
]}

机构：

[1] Russian Acad Med Sci, Gamaleya Res Inst Epidemiol & Microbiol, Moscow 123098, Russia

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1998年 / 401卷 / 1-2期

关键词：

UV-light induction; deletion; induced precise excision of transposon; Tn1; transposon; Tn5; Tn10; flanking inverted repeat; targeted and untargeted SOS-mutability; SOS-replication; SOS-regulon; recA mutant; lexA mutant; umuC mutant; Escherichia coli B; Escherichia coli K-12;

D O I：

10.1016/S0027-5107(98)00007-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

UV-inducible precise excision of transposons is a specific SOS-mutagenesis process. It deals with the deletion formation which has previously been demonstrated to involve direct or inverted IS-sequences of transposons. The process was used for revisiting the targeted and untargeted SOS-mutability and its relationship to the key genes for SOS-mutagenesis: the recA, lexA and umuDC. The precise excision of transposons Tn5 and Tn10 from the chromosomal insertion sites ade128 and cyc750 is induced in Escherichia coli K-12 and B cells, wild-type for DNA-repair, both by the low doses of UV-light ranging from 0.25 J m(-2) to 2.5 J m(-2) and the high doses within the range 5.0-40.0 J m(-2). Precise excision of these transposons induced by the range of low doses incapable to induce targeted point mutations reveals its mostly untargeted nature. This process for the transposon Tn1 is not induced by UV-light within the range of doses 0.25-2.5 J m(-2) while its induction is possible by UV-fluences ranging from 5.0 to 40.0 J m(-2). A dose-response of the precise excision of Tn1 is similar to that of the UV-induced reversion of trp,,, point mutation that is targeted by nature and contrasts to the UV-inducible precise excision of Tn5 and Tn10. Both types of UV-inducible precise excision, demonstrated either by Tn1 or Tn5 and Tn10, are eliminated by mutations in the lexA, recA and umuDC genes indispensable for UV-induced SOS-mutability. The palindromic structures different for the transposons Tn1, Tn5 and Tn10 are discussed to be involved and affect the targeted and untargeted precise excision of transposons induced by UV-light. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：179 / 191

页数：13

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