A determinant for directionality of organelle transport in Drosophila embryos

被引:76
作者
Gross, SP
Guo, Y
Martinez, JE
Welte, MA
机构
[1] Brandeis Univ, Rosenstiel Biomed Res Ctr, Waltham, MA 02454 USA
[2] Univ Calif Irvine, Dept Dev & Cell Biol, Irvine, CA 92697 USA
[3] Univ Calif Irvine, Dept Biomed Engn, Rockwell Engn Ctr 204, Irvine, CA 92697 USA
[4] Brandeis Univ, Dept Biol, Waltham, MA 02454 USA
[5] Brandeis Univ, Dept Biochem, Waltham, MA 02454 USA
关键词
D O I
10.1016/j.cub.2003.08.032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Motor-driven transport along microtubules is a primary cellular mechanism for moving and positioning organelles. Many cargoes move bidirectionally by using both minus and plus end-directed motors. How such cargoes undergo controlled net transport is unresolved. Results: Using a combination of genetics, molecular biology, and biophysics, we have identified Halo, a novel regulator of lipid droplet transport in early Drosophila embryos. In embryos lacking Halo, net transport of lipid droplets, but not that of other cargoes, is specifically altered; net transport is minus-end directed at developmental stages when it is normally plus-end directed. This reversal is due to an altered balance of motion at the level of individual organelles; without Halo, travel distances and stall forces are reduced for plus-end and increased for minus-end motion. During development, halo mRNA is highly upregulated just as net plus-end transport is initiated (phase 11), and its levels drop precipitously shortly before transport becomes minus-end directed (phase 111). Exogenously provided Halo prevents the switch to net minus-end transport in phase III in wild-type embryos and induces net plus-end transport during phase 11 in halo mutant embryos. This mechanism of regulation is likely to be of general importance because the Drosophila genome encodes a family of related proteins with similar sequences, each transiently expressed in distinct domains. Conclusions: We conclude that Halo acts as a directionality determinant for embryonic droplet transport and is the first member of a new class of transport regulators.
引用
收藏
页码:1660 / 1668
页数:9
相关论文
共 19 条
[1]   The balance of power in RNA trafficking [J].
Carson, JH ;
Cui, HY ;
Barbarese, E .
CURRENT OPINION IN NEUROBIOLOGY, 2001, 11 (05) :558-563
[2]   Dynactin is required for bidirectional organelle transport [J].
Deacon, SW ;
Serpinskaya, AS ;
Vaughan, PS ;
Fanarraga, ML ;
Vernos, I ;
Vaughan, KT ;
Gelfand, VI .
JOURNAL OF CELL BIOLOGY, 2003, 160 (03) :297-301
[3]   Asymmetric cell division: fly neuroblast meets worm zygote [J].
Doe, CQ ;
Bowerman, B .
CURRENT OPINION IN CELL BIOLOGY, 2001, 13 (01) :68-75
[4]   CELL-CYCLE CONTROL BY THE NUCLEOCYTOPLASMIC RATIO IN EARLY DROSOPHILA DEVELOPMENT [J].
EDGAR, BA ;
KIEHLE, CP ;
SCHUBIGER, G .
CELL, 1986, 44 (02) :365-372
[5]   BIDIRECTIONAL TRANSPORT OF FLUORESCENTLY LABELED VESICLES INTRODUCED INTO EXTRUDED AXOPLASM OF SQUID LOLIGO-PEALEI [J].
GILBERT, SP ;
SLOBODA, RD .
JOURNAL OF CELL BIOLOGY, 1984, 99 (02) :445-452
[6]   Coordination of opposite-polarity microtubule motors [J].
Gross, SP ;
Welte, MA ;
Block, SM ;
Wieschaus, EF .
JOURNAL OF CELL BIOLOGY, 2002, 156 (04) :715-724
[7]   Dynein-mediated cargo transport in vivo: A switch controls travel distance [J].
Gross, SP ;
Welte, MA ;
Block, SM ;
Wieschaus, EF .
JOURNAL OF CELL BIOLOGY, 2000, 148 (05) :945-955
[8]   Dynactin: Coordinating motors with opposite inclinations [J].
Gross, SP .
CURRENT BIOLOGY, 2003, 13 (08) :R320-R322
[9]   Interactions and regulation of molecular motors in Xenopus melanophores [J].
Gross, SP ;
Tuma, MC ;
Deacon, SW ;
Serpinskaya, AS ;
Reilein, AR ;
Gelfand, VI .
JOURNAL OF CELL BIOLOGY, 2002, 156 (05) :855-865
[10]  
Hollenbeck Peter J., 1996, Frontiers in Bioscience (online), V1, pD91