A non-invasive method for the tight anchoring of cells for scanning force microscopy

被引：8

作者：

Schilcher, K ^{[1
]}

Hinterdorfer, P ^{[1
]}

Gruber, HJ ^{[1
]}

Schindler, H ^{[1
]}

机构：

[1] Univ Linz, Inst Biophys, A-4040 Linz, Austria

来源：

CELL BIOLOGY INTERNATIONAL | 1997年 / 21卷 / 11期

关键词：

D O I：

10.1006/cbir.1997.0221

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Use of scanning force microscopy (SFM) for high resolution imaging of cell surfaces requires the cells to be tightly attached to substrates. Imaging of loosely adhered RBL-2H3 cells enabled determination of the cell size and investigation of larger structures and pseudopodia but failed in resolving more detail. Immobilization under non-invasive conditions via flexible crosslinkers containing a hydrophobic anchoring group enhanced resolution enormously. The cells were tightly attached to the substrates and were not removed by shear forces up to 80 nN as determined in a flow through apparatus. Morphological structures and dynamic processes on cell surfaces were observed as well as structural changes after cell stimulation upon ionomycin treatment. Molecular or atomic resolution, however, was not attainable which is attributed to the displacement of the flexible cell surface due to shear forces arising from the scanning tip during contact mode. (C) 1997 Academic Press.

引用

页码：769 / 778

页数：10

共 28 条

[1] IGE-INDUCED HISTAMINE-RELEASE FROM RAT BASOPHILIC LEUKEMIA-CELL LINES - ISOLATION OF RELEASING AND NON-RELEASING CLONES [J].