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Human immune response to sand fly salivary gland antigens: A useful epidemiological marker?
被引:129
作者:
Barral, A
Honda, E
Caldas, A
Costa, J
Vinhas, V
Rowton, ED
Valenzuela, JG
Charlab, R
Barral-Netto, M
Ribeiro, JMC
机构:
[1] NIAID, Sect Med Entomol, Parasit Dis Lab, Bethesda, MD 20892 USA
[2] FIOCRUZ, Ctr Pesquisas Goncalo Moniz, BR-40295001 Salvador, BA, Brazil
[3] Univ Fed Bahia, Hosp Univ Prof Edgard Santos, Serv Imunol, BR-40110040 Salvador, BA, Brazil
[4] Univ Fed Maranhao, Dept Patol, Sao Luiz, Maranhao, Brazil
[5] Walter Reed Army Inst Res, Dept Entomol, Bethesda, MD 20892 USA
关键词:
D O I:
10.4269/ajtmh.2000.62.740
中图分类号:
R1 [预防医学、卫生学];
学科分类号:
1004 ;
120402 ;
摘要:
Antibody (IgG) responses to salivary gland homogenate and to a recombinant salivary protein from the sand fly Lutzomyia longipalpis were investigated using sera from children living in an endemic area of visceral leishmaniasis in Brazil. We classified children into four groups according to their responses to Leishmania antigen: (Group I) positive serology and positive delayed type hypersensitivity (DTH), (Group II) positive serology and negative DTH, (Croup III) negative serology and positive DTH, and (Group IV) negative serology and negative DTH. A highly significant correlation was found between anti-salivary gland IgG levels and DTH responses. An L. longipalpis salivary recombinant protein used as an antigen in an enzyme-linked immune sorbent assay (ELISA) gave a significant but different result. A positive correlation was found between anti-leishmania IgG and anti-recombinant protein IgG titers. The results indicate that sand fly salivary proteins may be of relevance to the study the epidemiology of leishmaniasis.
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页码:740 / 745
页数:6
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