Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head

被引:254
作者
Nakata, T [1 ]
Hirokawa, N [1 ]
机构
[1] Univ Tokyo, Grad Sch Med, Dept Cell Biol & Anat, Tokyo 1130033, Japan
关键词
critical angle fluorescent microscopy; paclitaxel; polarized sorting; FB1; neurons;
D O I
10.1083/jcb.200302175
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Post-Golgi carriers of various newly synthesized axonal membrane proteins, which possess kinesin (KIF5)-driven highly processive motility, were transported from the TGN directly to axons. We found that KIF5 has a preference to the microtubules in the initial segment of axon. Low dose paclitaxel treatment caused missorting of KIF5, as well as axonal membrane proteins to the tips of dendrites. Microtubules in the initial segment of axons showed a remarkably high affinity to EB1-YFp, which was known to bind the tips of growing microtubules. These findings revealed unique features of the microtubule cytoskeletons in the initial segment, and suggested that they provide directional information for polarized axonal transport.
引用
收藏
页码:1045 / 1055
页数:11
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