Isolation of a small chitinase-like antifungal protein from Panax notoginseng (sanchi ginseng) roots

被引：64

作者：

Lam, SK ^{[1
]}

Ng, TB ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2001年 / 33卷 / 03期

关键词：

antifungal protein; chitinase; Panax notoginseng;

D O I：

10.1016/S1357-2725(01)00002-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antifungal protein, with a molecular weight of 15 kDa and an N-terminal sequence analogous to those of chitinases, was first isolated from the Chinese medicinal material Panax notoginseng, using cation exchange chromatography and affinity chromatography. The protein was adsorbed on CM-cellulose, Affi-gel Blue Gel and Mono S. It exerted antifungal activity against Coprinus comatus, Fusarium oxysporum and Mycosphaerella arachidicola but not against Rhizoctonia solani. The protein was devoid of ribonuclease activity against yeast tRNA. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：287 / 292

页数：6

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