Symbiotic mutants deficient in nodule establishment identified after T-DNA transformation of Lotus japonicus

被引:153
作者
Schauser, L [1 ]
Handberg, K [1 ]
Sandal, N [1 ]
Stiller, J [1 ]
Thykjær, T [1 ]
Pajuelo, E [1 ]
Nielsen, A [1 ]
Stougaard, J [1 ]
机构
[1] Aarhus Univ, Dept Biol Mol & Struct, Gene Express Lab, DK-8000 Aarhus C, Denmark
来源
MOLECULAR AND GENERAL GENETICS | 1998年 / 259卷 / 04期
关键词
root nodule development; T-DNA tagging; legume genetics; symbiotic loci; plant-microbe interaction;
D O I
10.1007/s004380050831
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nitrogen-fixing root nodules develop on legumes as a result of an interaction between host plants and soil bacteria collectively referred to as rhizobia. The organogenic process resulting in nodule development is triggered by the bacterial microsymbiont, but genetically controlled by the host plant genome. Using T-DNA insertion as a tool to identify novel plant genes that regulate nodule ontogeny, we have identified two putatively tagged symbiotic loci, Ljsym8 and Ljsym13, in the diploid legume Lotus japonicus. The sym8 mutants are arrested during infection by the bacteria early in the developmental process. The sym13 mutants are arrested in the final stages of infection, and ineffective nodules are formed. These two plant mutant lines were identified in progeny from 1112 primary transformants obtained after Agrobacterium tumefaciens T-DNA-mediated transformation of L. japonicus and subsequent screening for defects in the symbiosis with Mesorhizobium loti. Additional nontagged mutants arrested at different developmental stages were also identified and genetic complementation tests assigned all the mutations to IG monogenic symbiotic loci segregating recessive mutant alleles. In the screen reported here independent symbiotic loci thus appeared with a frequency of similar to 1.5%, suggesting that a relatively large set of genes is required for the symbiotic interaction.
引用
收藏
页码:414 / 423
页数:10
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