Vertical profiles of water temperature (n = 7193) and dissolved oxygen (n = 6516) were collected from 235 Missouri reservoirs during 1989-2007; most data were collected during May-August and provide a regional summary of summer conditions. Collectively, surface water temperature ranged from a mean of 22 C in May to 28 C in July, and individual summer maxima typically were 28-32 C. Most (95%) reservoirs stably stratify by mid-May, but few are deep enough to have hypolimnia with near-uniform temperatures. Among stratified reservoirs, maximum effective length and maximum depth accounted for 75% of the variation in mixed depth and thermocline depth. Ephemeral, near-surface thermoclines occurred in 39% of summer profiles and were most frequent in small, turbid reservoirs. Isotherms below the mixed layer deepen during stratification, and the water column is 20 C by August in all but the deepest reservoirs. Most reservoirs showed incipient dissolved oxygen (DO) depletion by mid-May, and by August, 80% of profiles had DO minima of 1 mg/L. Surface area and chlorophyll (Chl) explained 37% of variation in the earliest date of anoxia, and Chl explained 50% of variation in DO below the mixed layer during summer. Warm summer temperatures and widespread low DO often limit available fish habitat in Missouri reservoirs and compress warm-water fish communities into subsurface layers that exceed their thermal preferences. This study provides a regional baseline of reservoir temperature and oxygen conditions useful for future evaluations of eutrophication and the effects of a warming climate.