Biosensors as new analytical tool for detection of Genetically Modified Organisms (GMOs)

被引：57

作者：

Minunni, M

Tombelli, S

Mariotti, E

Mascini, M

机构：

[1] Univ Florence, Dipartimento Chim, I-50121 Florence, Italy

[2] Univ Teramo, Fac Agr, Mosciano Staz, TE, Italy

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 369卷 / 7-8期

关键词：

Nucleic Acid; Analytical Tool; Electrochemical Method; Target Analyte; Sensor Surface;

D O I：

10.1007/s002160100761

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Three different biosensors for detection of Genetically Modified Organisms (GMOs) are presented. The sensing principle is based on the affinity interaction between nucleic acids: the probe is immobilised on the sensor surface and the target analyte is free in solution, The immobilised probes are specific for most inserted sequences in GMOs: the promoter P35S and the terminator TNOS. Electrochemical methods with screen-printed electrodes, piezoelectric and optical (SPR) transduction principles were applied.

引用

页码：589 / 593

页数：5

共 5 条

[1] DISPOSABLE RUTHENIZED SCREEN-PRINTED BIOSENSORS FOR PESTICIDES MONITORING
CAGNINI, A
PALCHETTI, I
LIONTI, I
MASCINI, M
TURNER, APF
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1995, 24 (1-3) : 85 - 89
[2] Lipp M, 1999, J AOAC INT, V82, P923
[3] Disposable DNA electrochemical sensor for hybridization detection
Marrazza, G
Chianella, I
Mascini, M
[J]. BIOSENSORS & BIOELECTRONICS, 1999, 14 (01) : 43 - 51
[4] ADSORBED OMEGA-HYDROXY THIOL MONOLAYERS ON GOLD ELECTRODES - EVIDENCE FOR ELECTRON-TUNNELING TO REDOX SPECIES IN SOLUTION
MILLER, C
CUENDET, P
GRATZEL, M
[J]. JOURNAL OF PHYSICAL CHEMISTRY, 1991, 95 (02) : 877 - 886
[5] Coupling of a DNA piezoelectric biosensor and polymerase chain reaction to detect apolipoprotein E polymorphisms
Tombelli, S
Mascini, R
Braccini, L
Anichini, M
Turner, APF
[J]. BIOSENSORS & BIOELECTRONICS, 2000, 15 (7-8) : 363 - 370

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