A method for simultaneous detection and identification of Brazilian dog- and vampire bat-related rabies virus by reverse transcription loop-mediated isothermal amplification assay

被引：21

作者：

Saitou, Yasumasa ^{[1
]}

Kobayashi, Yuki ^{[1
,2
,3
]}

Hirano, Shinji ^{[1
]}

Mochizuki, Nobuyuki ^{[1
]}

Itou, Takuya ^{[1
]}

Ito, Fumio H. ^{[4
]}

Sakai, Takeo ^{[1
]}

机构：

[1] Nihon Univ, Vet Res Ctr, Kanagawa 2520880, Japan

[2] Natl Inst Genet, Ctr Informat Biol, Mishima, Shizuoka 4118540, Japan

[3] Natl Inst Genet, DNA Data Bank Japan, Mishima, Shizuoka 4118540, Japan

[4] Univ Sao Paulo, Dept Prevent Vet Med & Anim Hlth, Fac Vet Med & Zootechny, BR-05508000 Sao Paulo, Brazil

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 168卷 / 1-2期

关键词：

Brazil; Dog; RT-LAMP; Rabies; Vampire bat; RAPID DETECTION; FECAL SPECIMENS; NOROVIRUS; GENOME;

D O I：

10.1016/j.jviromet.2010.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

At present, the sporadic occurrence of human rabies in Brazil can be attributed primarily to dog- and vampire bat-related rabies viruses. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was employed as a simultaneous detection method for both rabies field variants within 60 min. Vampire bat-related rabies viruses could be distinguished from dog variants by digesting amplicons of the RT-LAMP reaction using the restriction enzyme Alwl. Amplification and digestion could both be completed within 120 min after RNA extraction. In addition, the RI-LAMP assay also detected rabies virus in isolates from Brazilian frugivorous bats and Ugandan dog, bovine and goat samples. In contrast, there were false negative results from several Brazilian insectivorous bats and all of Chinese dog, pig, and bovine samples using the RI-LAMP assay. This study showed that the RT-LAMP assay is effective for the rapid detection of rabies virus isolates from the primary reservoir in Brazil. Further improvements are necessary so that the RT-LAMP assay can be employed for the universal detection of genetic variants of rabies virus in the field. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：13 / 17

页数：5

共 18 条

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