Cultured motor neurons possess calcium-permeable AMPA/kainate receptors

被引：64

作者：

Bar-Peled, O

O'Brien, RJ

Morrison, JH

Rothstein, JD

机构：

[1] Johns Hopkins Univ, Dept Neurol, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Dept Neurosci, Baltimore, MD 21287 USA

[3] Mt Sinai Sch Med, Dept Neurobiol, New York, NY USA

来源：

NEUROREPORT | 1999年 / 10卷 / 04期

关键词：

ALS; Ca2+-permeable AMPA/kainate receptors; GluR2; motor neurons;

D O I：

10.1097/00001756-199903170-00034

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

WE examined the biology of AMPA/kainate-induced motor neuron degeneration using dissociated spinal cord cultures and motor neuron-specific antibodies which enable characterization of individual motor neurons in culture. Cobalt, which is thought to pass through Ca2+-permeable AMPA/kainate receptors following kainate exposure, labeled motor neurons in spinal cord cultures. The analysis of AMPA subunit distribution in dissociated motor neurons revealed a unique pattern of glutamate receptor (GluR) subunits in those cells; the GluR1 subunit was found in all spinal cord neurons, but the GluR2 subunit was not found in identified dissociated motor neurons. These data suggest that selective sensitivity of motor neurons to non-NMDA receptor activation is due, at least in part, to the presence of Ca2+-permeable AMPA/kainate receptors. NeuroReport 10:855-859 (C) 1999 Lippincott Williams & Wilkins.

引用

页码：855 / 859

页数：5

共 25 条

[1] Constitutive overexpression of Cu/Zn superoxide dismutase exacerbates kainic acid-induced apoptosis of transgenic-Cu/Zn superoxide dismutase neurons [J].