Applications of 1.06-μm IR laser desorption on a Fourier transform mass spectrometer

被引:36
作者
Ho, YP [1 ]
Fenselau, C [1 ]
机构
[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA
关键词
D O I
10.1021/ac980914s
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Various sugars, peptides, and lipids were analyzed on a Fourier transform mass spectrometer using laser desorption and ionization with and without the assistance of matrixes. A compact Nd:YAG laser with an output at 1.06 pm corresponding to fundamental frequency was employed. Gram-negative and Gram-positive bacteria were also subjected to laser desorption mass spectrometry. Characteristic ions of conjugated lipid, formed by attachment of alkali metal cations, endogenous to the cells, were observed. Particle/liquid matrixes (e.g., cobalt in glycerol) proved to be useful with the 1.06-mu m laser. The particles absorb efficiently laser radiation in a broad wavelength range. The liquid provides the same advantages as in fast atom bombardment: increased signal-to-noise ratios and enhanced sample lifetimes. The effect of laser power on total ion current was shown to differ for samples with and without the particle/liquid matrix. The Fourier transform analyzer provides MS/MS capability for both positive and negative ions from complex mixtures. Ions desorbed externally are introduced into the cell via a quadrupole ion guide with a lower mass cutoff. Such a setup allows matrix ions to be excluded and thus provides excellent signal-to-noise ratios for lower mass range fragment ions formed inside the cell.
引用
收藏
页码:4890 / 4895
页数:6
相关论文
共 37 条
[1]   4-SECTOR TANDEM MASS-SPECTROMETRIC ANALYSIS OF COMPLEX-MIXTURES OF PHOSPHATIDYLCHOLINES PRESENT IN A HUMAN-IMMUNODEFICIENCY-VIRUS PREPARATION [J].
BRYANT, DK ;
ORLANDO, RC ;
FENSELAU, C ;
SOWDER, RC ;
HENDERSON, LE .
ANALYTICAL CHEMISTRY, 1991, 63 (11) :1110-1114
[2]  
COLE MJ, 1994, ACS SYM SER, V541, P36
[3]   LIQUID-MIXTURES FOR MATRIX-ASSISTED LASER-DESORPTION [J].
CORNETT, DS ;
DUNCAN, MA ;
AMSTER, IJ .
ANALYTICAL CHEMISTRY, 1993, 65 (19) :2608-2613
[4]  
COTTER RJ, 1987, ANAL CHIM ACTA, V195, P45
[5]   Graphite/liquid mixed matrices for laser desorption/ionization mass spectrometry [J].
Dale, MJ ;
Knochenmuss, R ;
Zenobi, R .
ANALYTICAL CHEMISTRY, 1996, 68 (19) :3321-3329
[6]  
Dale R, 1997, RAPID COMMUN MASS SP, V11, P136
[7]  
DAVES GD, 1980, ADV MASS SPECTROM A, V8, P1012
[8]   DETERMINATION OF MOLECULAR-WEIGHT DISTRIBUTIONS OF POLYMERS BY MALDI-FTMS [J].
DEY, M ;
CASTORO, JA ;
WILKINS, CL .
ANALYTICAL CHEMISTRY, 1995, 67 (09) :1575-1579
[9]   MALDI MS and strategies for protein analysis [J].
Fenselau, C .
ANALYTICAL CHEMISTRY, 1997, 69 (21) :A661-A665
[10]  
FENSELAU C, 1994, ACS SYM SER, V541, P1