A multifunctional, synthetic Gaussia princeps luciferase reporter for live imaging of Candida albicans infections

被引：21

作者：

d'Enfert, Christophe ^{[1
,2
]}

Vecchiarelli, Anna ^{[3
]}

Brown, Alistair J. P. ^{[4
]}

机构：

[1] Inst Pasteur, Unite Biol & Pathogenicite Fong, Paris, France

[2] INRA, USC2019, Paris, France

[3] Univ Perugia, Dept Expt Med & Biochem Sci, Microbiol Sect, I-06100 Perugia, Italy

[4] Univ Aberdeen, Sch Med Sci, Aberdeen, Scotland

来源：

VIRULENCE | 2010年 / 1卷 / 03期

关键词：

luciferase; Gaussia princeps; Candida; candidiasis; Aspergillus; aspergillosis; imaging; reporter; gene fusion; host-pathogen interaction; IN-VIVO; FIREFLY LUCIFERASE; EXPRESSION; GENE;

D O I：

10.4161/viru.1.3.11119

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Novel luciferase reporters have been developed that allow real-time monitoring of infections by the fungal pathogens Candida albicans and Aspergillus fumigatus. Although these reporters still suffer limitations in the context of invasive infections, they provide unprecedented tools to monitor superficial infections and the efficacy of antifungal drugs or vaccines. In particular, the sensitivity and ease of detection of the cell-surface Gaussia princeps luciferase developed for C. albicans should make it a powerful tool for functional genomics studies in this and other pathogenic fungi.

引用

页码：174 / 176

页数：3

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