In situ crystallization of bacterial cellulose - III. Influences of different polymeric additives on the formation of microfibrils as revealed by transmission electron microscopy

被引:81
作者
Hirai, A
Tsuji, M
Yamamoto, H
Horii, F
机构
[1] Fukui Natl Coll Technol, Fukui 9160064, Japan
[2] Kyoto Univ, Chem Res Inst, Kyoto 6110011, Japan
关键词
bacterial cellulose; transmission electron microscopy; cellulose I-alpha; cellulose I-beta; microfibrils; carboxymethyl cellulose;
D O I
10.1023/A:1009233323237
中图分类号
TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];
学科分类号
0805 ; 080502 ; 0822 ;
摘要
Effects of polymer additives on the formation of microfibrils of bacterial cellulose have been examined by transmission electron microscopy, Among additives with different degrees of polymerization (DP) or substitution (DS), carboxymethyl cellulose sodium salt (CMC) with DP = 80 and DS = 0.57 is the most effective in producing separate, smaller-size microfibrils. By increasing the concentration of this CMC from 0.1 to 1.5%, the percentage of microfibrils measuring 3-7 nm wide is increased and levels off at around 1.0%. Other polymer additives such as xyloglucan are less effective than CMC in producing microfibrils with smaller sizes and the resulting microfibrils still tend to aggregate. The number of charged substituents and the molecular weight seem to be important factors in the production of highly separate smaller-size microfibrils. The reduction in average microfibril size is well correlated to the decrease in mass fraction of cellulose I-alpha in bacterial cellulose crystals. On the basis of these results, the mechanism of the crystallization of celluloses I-alpha and I-beta is discussed, The effect of colony types, smooth and rough, on the formation of microfibrils in the presence of CMC is also described. 0969-0239 (C) 1998 Blackie Academic & Professional.
引用
收藏
页码:201 / 213
页数:13
相关论文
共 20 条
[1]  
ATALLA RH, 1984, SCIENCE, V223, P283, DOI 10.1126/science.223.4633.283
[2]   SYNTHESIS OF CELLULOSE BY ACETOBACTER XYLINUM .8. ON FORMATION AND ORIENTATION OF BACTERIAL CELLULOSE FIBRILS IN PRESENCE OF ACIDIC POLYSACCHARIDES [J].
BENHAYYIM, G ;
OHAD, I .
JOURNAL OF CELL BIOLOGY, 1965, 25 (2P2) :191-+
[3]   CELLULOSE BIOSYNTHESIS IN ACETOBACTER-XYLINUM - VISUALIZATION OF SITE OF SYNTHESIS AND DIRECT MEASUREMENT OF INVIVO PROCESS [J].
BROWN, RM ;
WILLISON, JHM ;
RICHARDSON, CL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (12) :4565-4569
[4]  
Haigler C., 1982, CELLULOSE OTHER NATU, P273
[5]   ALTERATION OF INVIVO CELLULOSE RIBBON ASSEMBLY BY CARBOXYMETHYLCELLULOSE AND OTHER CELLULOSE DERIVATIVES [J].
HAIGLER, CH ;
WHITE, AR ;
BROWN, RM ;
COOPER, KM .
JOURNAL OF CELL BIOLOGY, 1982, 94 (01) :64-69
[6]   ELECTRON-DIFFRACTION ANALYSIS OF THE ALTERED CELLULOSE SYNTHESIZED BY ACETOBACTER-XYLINUM IN THE PRESENCE OF FLUORESCENT BRIGHTENING AGENTS AND DIRECT DYES [J].
HAIGLER, CH ;
CHANZY, H .
JOURNAL OF ULTRASTRUCTURE AND MOLECULAR STRUCTURE RESEARCH, 1988, 98 (03) :299-311
[7]  
HAIGLER CH, 1991, BIOSYNTHESIS BIODEGR, P99
[8]  
HAIGLER CH, 1989, CELLULOSE WOOD CHEM, P493
[9]   PEA XYLOGLUCAN AND CELLULOSE .5. XYLOGLUCAN-CELLULOSE INTERACTIONS INVITRO AND INVIVO [J].
HAYASHI, T ;
MARSDEN, MPF ;
DELMER, DP .
PLANT PHYSIOLOGY, 1987, 83 (02) :384-389
[10]   SYNTHESIS OF CELLULOSE BY ACETOBACTER-XYLINUM .2. PREPARATION OF FREEZE-DRIED CELLS CAPABLE OF POLYMERIZING GLUCOSE TO CELLULOSE [J].
HESTRIN, S ;
SCHRAMM, M .
BIOCHEMICAL JOURNAL, 1954, 58 (02) :345-352