Role of the regulatory gene rirA in the transcriptional response of Sinorhizobium meliloti to iron limitation

被引:70
作者
Chao, TC [1 ]
Buhrmester, J [1 ]
Hansmeier, N [1 ]
Pühler, A [1 ]
Weidner, S [1 ]
机构
[1] Univ Bielefeld, Fak Biol, Lehrstuhl Genet, D-33501 Bielefeld, Germany
关键词
D O I
10.1128/AEM.71.10.5969-5982.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A regulatory network of Sinorhizobium meliloti genes involved in adaptation to iron-limiting conditions and the involvement of the rhizobial iron regulator gene (rirA) were analyzed by mutation and microarray analyses. A constructed S. meliloti rirA mutant exhibited growth defects and enhanced H2O2 sensitivity in the presence of iron, but symbiotic nitrogen fixation was not affected. To identify iron-responsive and RirA-regulated S. meliloti genes, a transcriptome approach using whole-genome microarrays was used. Altogether, 45 genes were found to be jointly derepressed by mutation of rirA and under different iron-limited conditions. As expected, a number of genes involved in iron transport (e.g., hmuPSTU, shmR, rhbABCDEF, rhtX, and rhtA) and also genes with predicted functions in energy metabolism (e.g., fixN3, fixP3, and qxtAB) and exopolysaccharide production (e.g., exoY and exoN) were found in this group of genes. In addition, the iron deficiency response of S. meliloti also involved rirA-independent expression changes, including repression of the S. meliloti flagellar regulon. Finally, the RirA modulon also includes genes that are not iron responsive, including a gene cluster putatively involved in Fe-S cluster formation (sufA, sufS, sufD, sufC, and sufB).
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页码:5969 / 5982
页数:14
相关论文
共 74 条
[1]   Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon [J].
Baichoo, N ;
Wang, T ;
Ye, R ;
Helmann, JD .
MOLECULAR MICROBIOLOGY, 2002, 45 (06) :1613-1629
[2]   Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid [J].
Barnett, MJ ;
Fisher, RF ;
Jones, T ;
Komp, C ;
Abola, AP ;
Barloy-Hubler, F ;
Bowser, L ;
Capela, D ;
Galibert, F ;
Gouzy, J ;
Gurjal, M ;
Hong, A ;
Huizar, L ;
Hyman, RW ;
Kahn, D ;
Kahn, ML ;
Kalman, S ;
Keating, DH ;
Palm, C ;
Peck, MC ;
Surzycki, R ;
Wells, DH ;
Yeh, KC ;
Davis, RW ;
Federspiel, NA ;
Long, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9883-9888
[3]   Identification of an iron-regulated, hemin-binding outer membrane protein in Sinorhizobium meliloti [J].
Battistoni, F ;
Platero, R ;
Duran, R ;
Cerveñansky, C ;
Battistoni, J ;
Arias, A ;
Fabiano, E .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) :5877-5881
[4]   Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor [J].
Beaumont, HJE ;
Lens, SI ;
Reijnders, WNM ;
Westerhoff, HV ;
van Spanning, RJM .
MOLECULAR MICROBIOLOGY, 2004, 54 (01) :148-158
[5]   Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions [J].
Becker, A ;
Bergès, H ;
Krol, E ;
Bruand, C ;
Rüberg, S ;
Capela, D ;
Lauber, E ;
Meilhoc, E ;
Ampe, F ;
de Bruijn, FJ ;
Fourment, J ;
Francez-Charlot, A ;
Kahn, D ;
Küster, H ;
Liebe, C ;
Pühler, A ;
Weidner, S ;
Batut, J .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2004, 17 (03) :292-303
[6]  
BERINGER JE, 1974, J GEN MICROBIOL, V84, P188
[7]   MOLECULAR-CLONING AND DNA-SEQUENCE ANALYSIS OF A DIPHTHERIA TOX IRON-DEPENDENT REGULATORY ELEMENT (DTXR) FROM CORYNEBACTERIUM-DIPHTHERIAE [J].
BOYD, J ;
OZA, MN ;
MURPHY, JR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5968-5972
[8]   TRANSPORT OF IRON ACROSS THE OUTER-MEMBRANE [J].
BRAUN, V ;
GUNTER, K ;
HANTKE, K .
BIOLOGY OF METALS, 1991, 4 (01) :14-22
[9]   Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021 [J].
Capela, D ;
Barloy-Hubler, F ;
Gouzy, J ;
Bothe, G ;
Ampe, F ;
Batut, J ;
Boistard, P ;
Becker, A ;
Boutry, M ;
Cadieu, E ;
Dréano, S ;
Gloux, S ;
Godrie, T ;
Goffeau, A ;
Kahn, D ;
Kiss, E ;
Lelaure, V ;
Masuy, D ;
Pohl, T ;
Portetelle, D ;
Pühler, A ;
Purnelle, B ;
Ramsperger, U ;
Renard, C ;
Thébault, P ;
Vandenbol, M ;
Weidner, S ;
Galibert, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9877-9882
[10]   SIDEROPHORE AND ORGANIC-ACID PRODUCTION IN ROOT NODULE BACTERIA [J].
CARSON, KC ;
HOLLIDAY, S ;
GLENN, AR ;
DILWORTH, MJ .
ARCHIVES OF MICROBIOLOGY, 1992, 157 (03) :264-271