Identification of nucleotide sequences for the specific and rapid detection of Yersinia pestis

被引：44

作者：

Radnedge, L

Gamez-Chin, S

McCready, PM

Worsham, PL

Andersen, GL

机构：

[1] Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA

[2] USA, Res Inst Infect Dis, Ft Detrick, MD 21702 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 08期

关键词：

D O I：

10.1128/AEM.67.8.3759-3762.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Suppression subtractive hybridization, a cost-effective approach for targeting unique DNA, was used to identify a 41.7-kb Yersinia pestis-specific region. One primer pair designed from this region amplified PCR products from natural isolates of Y. pestis and produced no false positives for near neighbors, an important criterion for unambiguous bacterial identification.

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页码：3759 / 3762

页数：4

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