p53 inhibits entry into mitosis when DNA synthesis is blocked

被引：64

作者：

Taylor, WR ^{[1
]}

Agarwal, ML ^{[1
]}

Agarwal, A ^{[1
]}

Stacey, DW ^{[1
]}

Stark, GR ^{[1
]}

机构：

[1] Cleveland Clin Fdn, Lerner Res Inst, Dept Biol Mol, Cleveland, OH 44195 USA

来源：

ONCOGENE | 1999年 / 18卷 / 02期

关键词：

cell cycle; checkpoint; hydroxyurea; PALA;

D O I：

10.1038/sj.onc.1202516

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human and mouse fibroblasts with normal p53 fail to enter mitosis when DNA synthesis is blocked by aphidicolin or hydroxyurea, Isogenic p53-null fibroblasts do enter mitosis with incompletely replicated DNA, revealing that p53 contributes to a checkpoint that ensures that mitosis does not occur until DNA synthesis is complete, When treated with N-(phosphonacetyl)-L-aspartate (PALA), which inhibits pyrimidine nucleotide synthesis, leading to synthesis of damaged DNA from highly unbalanced dNTP pools, p53-null cells enter mitosis after they have completed DNA replication, but cells with wild-type p53 do not, revealing that p53 also mediates a checkpoint that monitors the quality of newly replicated DNA.

引用

页码：283 / 295

页数：13

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