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Transposition of MINE, a composite retrotransposon, in the avirulence gene ACE1 of the rice blast fungus Magnaporthe grisea

被引：69
作者：
Fudal, I
Böhnert, HU
Tharreau, D
Lebrun, MH [1 ]
机构：
[1] Bayer CropSci, CNRS, UMR2847, F-69263 Lyon, France
[2] CIRAD, ENSAM, INRA, UMR,BGPI, F-34398 Montpellier, France
来源：
FUNGAL GENETICS AND BIOLOGY | 2005年 / 42卷 / 09期
关键词：
Magnaporthe grisea; retrotransposon; avirulence; transposition; integration; reverse transcription; U6-L1; chimeras;
D O I：
10.1016/j.fgb.2005.05.001
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The ACE1 avirulence gene allele from the rice blast fungus Magnaporthe grisea was characterized in virulent isolate 2/0/3, revealing the insertion of a 1.9 kb MINE retrotransposon in the last ACE1 exon. MINE is a novel chimeric element composed of a transcribed non-coding sequence of 1.1 kb (WEIRD) fused to a 5'-truncated MGL retrotransposon. MINEs were found in high copy number in M. grisea isolates from rice (68 copies) and as a single copy in isolate CD156 from Eleusine. MINEs vary in size (1.3-6.7 kb) with conserved 5' WEIRD sequences and variable 3' MGL sequences. MGLs fused to WEIRDs correspond to different 5'-truncated MGLs with conserved 3' ends. The organization and diversity of MINEs suggest that these retrotransposons result from independent fusions between WEIRD and 5'-truncated MGLs. Such chimera could be formed during MGL reverse transcription as proposed for human U6-LINE1 chimeric retrotransposons and integrated into M. grisea genome using MGL machinery. (c) 2005 Elsevier Inc. All rights reserved.
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页码：761 / 772
页数：12
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