Heat shock protein 100 and the amastigote stage-specific A2 proteins of Leishmania donovani

被引：12

作者：

Clos, J

Klaholz, L

Kroemer, M

Krobitsch, S

Lindquist, S

机构：

[1] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

[2] Univ Chicago, Howard Hughes Med Inst, Chicago, IL 60637 USA

来源：

MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 2001年 / 190卷 / 1-2期

关键词：

D O I：

10.1007/s004300100078

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

HSP100 protein in Leishmania spp. plays an important role for the survival and integrity of intracellular amastigotes, The A2 proteins of L. donovani are functionally linked to HSP100. There is evidence for an interdependence between these two proteins, which are both expressed predominantly in the amastigote stage of Leishmania donovani. Mutant strains lacking either of these proteins display very similar phenotypes, i.e. loss of virulence both in vivo and in vitro. Also, both proteins colocalise specifically to small foci within the cytoplasm of amastigotes.

引用

收藏

页码：47 / 50

页数：4

相关论文

共 25 条

[1] Targeting of exogenous DNA into Trypanosoma brucei requires a high degree of homology between donor and target DNA [J].

Blundell, PA ;

Rudenko, G ;

Borst, P .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 76 (1-2) :215-229

[2] HIGH CONSTITUTIVE LEVELS OF HEAT-SHOCK PROTEINS IN HUMAN-PATHOGENIC PARASITES OF THE GENUS LEISHMANIA [J].

BRANDAU, S ;

DRESEL, A ;

CLOS, J .

BIOCHEMICAL JOURNAL, 1995, 310 :225-232

[3] DEVELOPMENTAL GENE-EXPRESSION IN LEISHMANIA-DONOVANI - DIFFERENTIAL CLONING AND ANALYSIS OF AN AMASTIGOTE-STAGE-SPECIFIC GENE [J].

CHAREST, H ;

MATLASHEWSKI, G .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) :2975-2984

[4] ROLE OF THE CHAPERONE PROTEIN HSP104 IN PROPAGATION OF THE YEAST PRION-LIKE FACTOR [PSI(+)] [J].

CHERNOFF, YO ;

LINDQUIST, SL ;

ONO, B ;

INGEVECHTOMOV, SG ;

LIEBMAN, SW .

SCIENCE, 1995, 268 (5212) :880-884

[5] DOUBLE TARGETED GENE REPLACEMENT FOR CREATING NULL MUTANTS [J].

CRUZ, A ;

COBURN, CM ;

BEVERLEY, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :7170-7174

[6] ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].

GARNIER, J ;

OSGUTHORPE, DJ ;

ROBSON, B .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120

[7] CONSERVATION OF THE REGULATORY SUBUNIT FOR THE CLP ATP-DEPENDENT PROTEASE IN PROKARYOTES AND EUKARYOTES [J].

GOTTESMAN, S ;

SQUIRES, C ;

PICHERSKY, E ;

CARRINGTON, M ;

HOBBS, M ;

MATTICK, JS ;

DALRYMPLE, B ;

KURAMITSU, H ;

SHIROZA, T ;

FOSTER, T ;

CLARK, WP ;

ROSS, B ;

SQUIRES, CL ;

MAURIZI, MR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) :3513-3517

[8] STABLE TRANSFORMATION OF TRYPANOSOMA-CRUZI - INACTIVATION OF THE PUB12.5-POLYUBIQUITIN GENE BY TARGETED GENE DISRUPTION [J].

HARIHARAN, S ;

AJIOKA, J ;

SWINDLE, J .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 57 (01) :15-30

[9]

Hubel A, 1997, MOL CELL BIOL, V17, P5987

[10] A MEMBER OF THE CLPB FAMILY OF STRESS PROTEINS IS EXPRESSED DURING HEAT-SHOCK IN LEISHMANIA SPP [J].

HUBEL, A ;

BRANDAU, S ;

DRESEL, A ;

CLOS, J .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 70 (1-2) :107-118