Cloning and characterization of mouse tec promoter

被引：12

作者：

Honda, H ^{[1
]}

Yamashita, Y ^{[1
]}

Ozawa, K ^{[1
]}

Mano, H ^{[1
]}

机构：

[1] JICHI MED SCH, INST HEMATOL, DEPT BIOL MOLEC, MINAMI KAWACHI, TOCHIGI 32904, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 223卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.0909

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tec is a cytoplasmic protein-tyrosine kinase highly expressed in hematopoietic precursor cells. To investigate the mechanism regulating its expression, we cloned and characterized the mouse tec promoter. The promoter region does not contain the authentic TATA or CAAT box but has several consensus binding motifs for GATA and SP1. The nucleotide sequence surrounding the major transcription initiation site determined by 5' RACE-PCR appeared to be homologous to the ''initiator'' consensus sequence which is frequently found in genes lacking TATA and CAAT. The promoter activity of the 5' flanking region was confirmed by using the luciferase assay. The present analysis will provide insights into the mechanism for the expression and regulation of the Tee molecule. (C) 1996 Academic Press, Inc.

引用

页码：422 / 426

页数：5

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