Aloe-emodin induces apoptosis in T24 human bladder cancer cells through the p53 dependent apoptotic pathway

被引:88
作者
Lin, JG
Chen, GW
Li, TM
Chouh, ST
Tan, TW
Chung, JG
机构
[1] China Med Univ, Dept Microbiol, Sch Med, Taichung 404, Taiwan
[2] China Med Univ, Dept Pharmacol, Taichung 404, Taiwan
[3] China Med Univ, Sch Chinese Med, Taichung 404, Taiwan
关键词
bladder; bladder neoplasms; aloe; emodin; apoptosis;
D O I
10.1016/S0022-5347(05)00005-4
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Purpose: We investigated the anticancer effect of AE(1,8-dihydroy-3-[hydroxymethyl]-anthraquione) in the T24 human bladder cancer cell line (Food Industry Research and Development Institute, Hsinchu, Taiwan) by studying apoptosis regulation. Materials and Methods: AE, which is purified from aloe vera leaves, has been reported to have antitumor activity. Cell viability, cell cycle and apoptosis were determined by flow cytometric methods. Levels of cyclins, cyclin-dependent kinase 1 and other enzyme were examined by Western blotting methods. Results: AE inhibited cell viability, and induced G2/M arrest and apoptosis in T24 cells. AE increased the levels of Wee1 and cdc25c, and may have led to inhibition of the levels of cyclin-dependent kinase 1 and cyclin 131, which cause G2/M arrest. AE induced p53 expression and was accompanied by the induction of p21 and caspase-3 activation, which was associated with apoptosis. In addition, AE was associated with a marked increase in Fas/APO1 receptor and Bax expression but it inhibited 136-2 expression. Conclusions: AE induced apoptosis in T24 cells is mediated through the activation of p53, p2l, Fas/APO-1, Bax and caspase-3.
引用
收藏
页码:343 / 347
页数:5
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