Threonine phosphorylation of modulator protein RsbR governs its ability to regulate a serine kinase in the environmental stress signaling pathway of Bacillus subtilis

被引:67
作者
Gaidenko, TA
Yang, XF
Lee, YM
Price, CW [1 ]
机构
[1] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA
[2] Univ Calif Davis, Prot Struct Lab, Davis, CA 95616 USA
关键词
covalent modification; environmental signaling phosphorylation; signal transduction; stress response;
D O I
10.1006/jmbi.1999.2665
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The sigma(B) transcription factor of the bacterium Bacillus subtilis controls the synthesis of over 100 general stress proteins that are induced by growth-limiting conditions. Genetic evidence suggests that RsbR modulates the phosphorylation state of the RsbS antagonist in the signaling pathway that regulates sigma(B) activity in response to environmental stresses that limit growth. According to the current model, the phosphorylated RsbS antagonist is unable to complex RsbT, which is then released to initiate a signaling cascade that ultimately activates sigma(B). Here, we show that the RsbR protein itself has no kinase activity but instead stimulates RsbS phosphorylation by the RsbT serine kinase in vitro. We further show that in addition to its previously known serine kinase activity directed toward the RsbS antagonist, purified RsbT also possesses a threonine kinase activity directed toward residues 171 and 205 of the RsbR modulator. Threonine residues 171 and 205 were each found to be important for RsbR function in vivo, and phosphorylation of these residues abolished the ability of RsbR to stimulate RsbT kinase activity in vitro. These results are consistent with a model in which RsbR modulates the kinase activity of RsbT directed toward its RsbS antagonist in vivo, either specifically in response to environmental signals or as part of a feedback mechanism to prevent continued signaling. (C) 1999 Academic Press.
引用
收藏
页码:29 / 39
页数:11
相关论文
共 38 条
  • [1] Modulator protein RsbR regulates environmental signalling in the general stress pathway of Bacillus subtilis
    Akbar, S
    Kang, CM
    Gaidenko, TA
    Price, CW
    [J]. MOLECULAR MICROBIOLOGY, 1997, 24 (03) : 567 - 578
  • [2] AN ADENOSINE NUCLEOTIDE SWITCH CONTROLLING THE ACTIVITY OF A CELL-TYPE-SPECIFIC TRANSCRIPTION FACTOR IN BACILLUS-SUBTILIS
    ALPER, S
    DUNCAN, L
    LOSICK, R
    [J]. CELL, 1994, 77 (02) : 195 - 205
  • [3] Role of adenosine nucleotides in the regulation of a stress-response transcription factor in Bacillus subtilis
    Alper, S
    Dufour, A
    Garsin, DA
    Duncan, L
    Losick, R
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1996, 260 (02) : 165 - 177
  • [4] ARBUTHNOTT JP, 1974, ISOELECTRIC FOCUSING, P212
  • [5] Identification of the gene encoding the alternative sigma factor σB from Listeria monocytogenes and its role in osmotolerance
    Becker, LA
    Çetin, MS
    Hutkins, RW
    Benson, AK
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (17) : 4547 - 4554
  • [6] BACILLUS-SUBTILIS SIGMA-B IS REGULATED BY A BINDING-PROTEIN (RSBW) THAT BLOCKS ITS ASSOCIATION WITH CORE RNA-POLYMERASE
    BENSON, AK
    HALDENWANG, WG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (06) : 2330 - 2334
  • [7] THE SIGMA-B-DEPENDENT PROMOTER OF THE BACILLUS-SUBTILIS SIGB OPERON IS INDUCED BY HEAT-SHOCK
    BENSON, AK
    HALDENWANG, WG
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (07) : 1929 - 1935
  • [8] Specific and general stress proteins in Bacillus subtilis - A two-dimensional protein electrophoresis study
    Bernhardt, J
    Volker, U
    Volker, A
    Antelmann, H
    Schmid, R
    Mach, H
    Hecker, M
    [J]. MICROBIOLOGY-UK, 1997, 143 : 999 - 1017
  • [9] ACTIVATION OF BACILLUS-SUBTILIS TRANSCRIPTION FACTOR SIGMA(B) BY A REGULATORY REGULATORY PATHWAY RESPONSIVE TO STATIONARY-PHASE SIGNALS
    BOYLAN, SA
    RUTHERFORD, A
    THOMAS, SM
    PRICE, CW
    [J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (11) : 3695 - 3706
  • [10] STRESS-INDUCED ACTIVATION OF THE SIGMA(B) TRANSCRIPTION FACTOR OF BACILLUS-SUBTILIS
    BOYLAN, SA
    REDFIELD, AR
    BRODY, MS
    PRICE, CW
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (24) : 7931 - 7937