Stability of oxidases immobilized in silica gels

被引:175
作者
Chen, Q [1 ]
Kenausis, GL [1 ]
Heller, A [1 ]
机构
[1] Univ Texas, Dept Chem Engn, Austin, TX 78712 USA
关键词
D O I
10.1021/ja971750k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The gain or loss of stability of three flavoprotein oxidases, glucose oxidase, lactate oxidase, and glycolate oxidase, upon immobilization in a hydrated silica gel by a sol-gel process was quantified. Glucose oxidase (isoelectric point, IP, pH 3.8) retained most or all of its initial activity, while lactate oxidase and glycolate lost most of theirs. The half-life of glucose oxidase at 63 degrees C increased upon immobilization 200-fold; the half-lives of lactate oxidase and of glycolate oxidase were not extended beyond those of the water-dissolved enzymes. After lactate oxidase (IP pH 4.6) was electrostatically complexed with the weak base poly(N-vinylimidazole) prior to its immobilization, most of its activity was retained and its half-life at 63 degrees C increased 150-fold. Lactate oxidase was also stabilized when electrostatically complexed with the stronger base poly(ethyleneimine) prior to immobilization. Glycolate oxidase (IP pH 9.6) was not stabilized by poly(N-vinylimidazole) but was stabilized by poly(ethyleneimine) complexing prior to immobilization. The complexed enzyme retained its initial activity upon immobilization, and its half-life at 60 degrees C also increased 100-fold. The results show that encaging an oxidase in a silica gel can lead either to gain in stability or to loss of activity and that electrostatic complexing is required for stabilization by encapsulation of some, but not all, flavoprotein oxidases.
引用
收藏
页码:4582 / 4585
页数:4
相关论文
共 27 条
  • [1] ATTIA YA, 1994, SOL GEL PROCESSING A
  • [2] ENZYMES AND OTHER PROTEINS ENTRAPPED IN SOL-GEL MATERIALS
    AVNIR, D
    BRAUN, S
    LEV, O
    OTTOLENGHI, M
    [J]. CHEMISTRY OF MATERIALS, 1994, 6 (10) : 1605 - 1614
  • [3] Avnir D., 1996, BIOCH ASPECTS SOL GE
  • [4] SYNTHESIS OF PROTEIN-DOPED SOL-GEL SIO2 THIN-FILMS - EVIDENCE FOR ROTATIONAL MOBILITY OF ENCAPSULATED CYTOCHROME-C
    DAVE, BC
    SOYEZ, H
    MILLER, JM
    DUNN, B
    VALENTINE, JS
    ZINK, JI
    [J]. CHEMISTRY OF MATERIALS, 1995, 7 (08) : 1431 - 1434
  • [5] SOL-GEL ENCAPSULATION METHODS FOR BIOSENSORS
    DAVE, BC
    DUNN, B
    VALENTINE, JS
    ZINK, JI
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (22) : A1120 - A1127
  • [6] THE INTERACTION OF POSITIVELY CHARGED POLYMER PARTICLES WITH WOOL FIBERS
    FLEISCHFRESSER, BE
    [J]. TEXTILE RESEARCH JOURNAL, 1982, 52 (05) : 328 - 334
  • [7] PRESERVATION OF SHELF-LIFE OF ENZYME-BASED ANALYTICAL SYSTEMS USING A COMBINATION OF SUGARS, SUGAR ALCOHOLS AND CATIONIC POLYMERS OF ZINC IONS
    GIBSON, TD
    HULBERT, JN
    WOODWARD, JR
    [J]. ANALYTICA CHIMICA ACTA, 1993, 279 (01) : 185 - 192
  • [8] EXTENDED SHELF-LIFE OF ENZYME-BASED BIOSENSORS USING A NOVEL STABILIZATION SYSTEM
    GIBSON, TD
    HULBERT, JN
    PARKER, SM
    WOODWARD, JR
    HIGGINS, IJ
    [J]. BIOSENSORS & BIOELECTRONICS, 1992, 7 (10) : 701 - 708
  • [9] Gupta M., 1993, Thermostability of Enzymes
  • [10] Stabilization of lactate oxidase in screen-printed enzyme electrodes
    Hart, AL
    Cox, H
    Janssen, D
    [J]. BIOSENSORS & BIOELECTRONICS, 1996, 11 (08) : 833 - 837