Glycolipids as receptors for Bacillus thuringiensis crystal toxin

被引：272

作者：

Griffitts, JS

Haslam, SM

Yang, TL

Garczynski, SF

Mulloy, B

Morris, H

Cremer, PS

Dell, A

Adang, MJ

Aroian, RV ^{[1
]}

机构：

[1] Univ Calif San Diego, Sect Cell & Dev Biol, La Jolla, CA 92093 USA

[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol Sci, London SW7 2AZ, England

[3] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA

[4] Univ Georgia, Dept Entomol, Athens, GA 30602 USA

[5] Natl Inst Biol Stand & Controls, Mol Struct Lab, Potters Bar EN6 3QG, Herts, England

[6] MSCAN Mass Spectrometry Res & Training Ctr, Ascot SL5 7PZ, Berks, England

来源：

SCIENCE | 2005年 / 307卷 / 5711期

关键词：

D O I：

10.1126/science.1104444

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The development of pest resistance threatens the effectiveness of Bacillus thuringiensis (Bt) toxins used in transgenic and organic farming. Here, we demonstrate that (i) the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates; (ii) Bt toxin directly and specifically binds glycotipids; and (iii) this binding is carbohydrate-dependent and relevant for toxin action in vivo. These carbohydrates contain the arthroseries core conserved in insects and nematodes but lacking in vertebrates. We present evidence that insect glycolipids are also receptors for Bt toxin.

引用

页码：922 / 925

页数：4

共 26 条

[1] Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests [J].