Characterization of novel psychrophilic clostridia from an Antarctic microbial mat:: description of Clostridium frigoris sp nov., Clostridium lacusfryxellense sp nov., Clostridium bowmanii sp nov and Clostridium psychrophilum sp nov and reclassification of Clostridium laramiense as Clostridium estertheticum subsp laramiense subsp nov.

Taxonomic studies were performed on four strains (D-1/D-an/IIT, C/C-an/B1(T), A-1/C-an/C1(T) and A-1/C-an/I-T) of anaerobic, Gram-positive, spore-forming bacteria originally isolated from a mat sample retrieved from a shallow, moated area around Lake Fryxell, an Antarctic freshwater lake. Phylogenetic analyses based on 16S rRNA gene sequence data indicated that these strains are affiliated with cluster I clostridia and form a coherent group with Clostridium estertheticum and Clostridium laramiense. Similarity values among 16S rRNA gene sequences within this assemblage ranged between 96.7 and 99.8%. Despite the close phylogenetic relationship, several distinguishing phenotypic traits were found among the novel strains using a polyphasic approach. All strains were psychrophilic, but the temperature optimum for growth differed markedly, ranging from 4 to 16 degreesC. In addition, substrate utilization patterns, fermentation end products, cellular fatty acid profiles and morphological traits enabled a clear differentiation between the strains. DNA-DNA hybridization experiments revealed that each of the four novel strains represents a distinct species, with DNA-DNA similarity values to related strains in the range 16-62%. In contrast, the type strains of C. estertheticum and C. laramiense shared 79% DNA-DNA similarity, indicating a close relationship at the species level. On the basis of genetic and phenotypic properties, it is proposed to designate four novel species of the genus Clostridium to harbour the newly isolated strains: Clostridium frigoris sp. nov. (type strain D-1/D-an/IIT = DSM 14204(T) = ATCC BAA-579(T)), Clostridium lacusfryxellense sp. nov. (type strain C/C-an/B1(T) = DSM 14205(T) = ATCC BAA-580(T)), Clostridium bowmanii sp. nov. (type strain A-1/C-an/C1(T) = DSM 14206(T) = ATCC BAA-581(T)) and Clostridium psychrophilum sp. nov. (type strain A-1/C-an/I-T = DSM 14207(T) = ATCC BAA-582(T)). It is also proposed to unite C. laramiense and C. estertheticum under C. estertheticum. The subspecies C. estertheticum subsp. laramiense subsp. nov. is established, represented by strain ATCC 51254 7 (= DSM 14864(T)). The type strain of C. estertheticum subsp. estertheticum remains NCIMB 125117 (= DSM 88097).