Diagnostic value of proteins of three Borrelia species (Borrelia burgdorferi sensu lato) and implications for development and use of recombinant antigens for serodiagnosis of Lyme borreliosis in Europe

被引:52
作者
Hauser, U [1 ]
Lehnert, G [1 ]
Wilske, B [1 ]
机构
[1] Univ Munich, Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany
关键词
D O I
10.1128/CDLI.5.4.456-462.1998
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
More and more assays for the serodiagnosis of Lyme borreliosis (LB) are based on recombinant antigens. However, so far, there is no consensus as to which are the most specific and sensitive proteins and how they should be used in combination to obtain tests with the best discrimination abilities. The present study was preceded by a detailed analysis of Western blots (WB) using whole-cell lysates of Borrelia burgdorferi sensu stricto strain PKa2, B. afzelii PKo, and B. garinii PBi (U. Hauser, G. Lehnert, R. Lobentanzer, and B. Wilske, J. Clin. Microbiol. 35:1433-1444, 1997). For the present work, the data bank from that study, containing information about the reactivities of 330 sera (from patients at different stages of LB [n = 189]; control group, n = 141), was reused. The specificities and sensitivities of various combinations of proteins from different strains were calculated for different interpretation criteria. For immunoglobulin G (IgG) WE, the recommended combination of antigens available to date as recombinant proteins included p83/100 of PKa2, p83/100 of PKo, p39 of PKo, p39 of PBI, and OspC of PBi (interpretation criterion, at least one reactive band required for a positive WE; specificity, 96.5%; sensitivity, 56.1%). The further addition of p58 of PKo, p17 of PKo, or p14 of PKo was most favorable in terms of both a considerable gain of sensitivity and little loss of specificity. IgG Western blotting with a whole-cell lysate of strain PKo might be improved by the addition of OspC of PBi. For IgG WE, the best combination, out of all bands, was p83/100, p58, p39, p30, and p21 of all three strains and OspC of PBI, p17b of PBi, p56 of PKa2, p43 of PKo, p17 of PKo, and p14 of PKo (interpretation criterion, at least two reactive bands required for a positive WB; specificity, 97.2%; sensitivity, 61.4%). An interpretation criterion of at least two reactive bands is more reliable than one of only one reactive band. For IgM WB, the best combination was OspC of PKo, OspC of PBi, p39 of all three strains, p17 of PKo, and strong reactions with p41 of all three strains (interpretation criterion, at least one reactive band required; specificity, 97.9%; sensitivity, 47.0%).
引用
收藏
页码:456 / 462
页数:7
相关论文
共 49 条
[1]  
*ASS STAT TERR PUB, 1995, P 2 NAT C SER DIAGN, P1
[2]   WESTERN-BLOT-ANALYSIS OF SERA FROM LYME BORRELIOSIS PATIENTS ACCORDING TO THE GENOMIC SPECIES OF THE BORRELIA STRAINS USED AS ANTIGENS [J].
ASSOUS, MV ;
POSTIC, D ;
PAUL, G ;
NEVOT, P ;
BARANTON, G .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1993, 12 (04) :261-268
[3]   DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS [J].
BARANTON, G ;
POSTIC, D ;
SAINTGIRONS, I ;
BOERLIN, P ;
PIFFARETTI, JC ;
ASSOUS, M ;
GRIMONT, PAD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (03) :378-383
[4]   LYME-DISEASE SPIROCHETES AND IXODID TICK SPIROCHETES SHARE A COMMON SURFACE ANTIGENIC DETERMINANT DEFINED BY A MONOCLONAL-ANTIBODY [J].
BARBOUR, AG ;
TESSIER, SL ;
TODD, WJ .
INFECTION AND IMMUNITY, 1983, 41 (02) :795-804
[5]   DIAGNOSIS OF LYME BORRELIOSIS BY AN ENZYME-IMMUNOASSAY DETECTING IMMUNOGLOBULIN-G REACTIVE TO PURIFIED BORRELIA-BURGDORFERI CELL COMPONENTS [J].
BERGSTROM, S ;
SJOSTEDT, A ;
DOTEVALL, L ;
KAIJSER, B ;
EKSTRANDHAMMARSTROM, B ;
WALLBERG, C ;
SKOGMAN, G ;
BARBOUR, AG .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1991, 10 (05) :422-427
[6]  
BUNIKIS J, 1995, FEMS MICROBIOL LETT, V131, P139, DOI 10.1111/j.1574-6968.1995.tb07768.x
[7]   Development of enzyme-linked immunosorbent assays using recombinant borrelial antigens for serodiagnosis of Borrelia burgdorferi infection [J].
Burkert, S ;
Rossler, D ;
Munchhoff, P ;
Wilske, B .
MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 1996, 185 (01) :49-57
[8]   CHARACTERIZATION OF ANTIGENIC DETERMINANTS OF BORRELIA-BURGDORFERI SHARED BY OTHER BACTERIA [J].
COLEMAN, JL ;
BENACH, JL .
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (04) :658-666
[9]   ANTIBODY-RESPONSES TO THE 3 GENOMIC GROUPS OF BORRELIA-BURGDORFERI IN EUROPEAN LYME BORRELIOSIS [J].
DRESSLER, F ;
ACKERMANN, R ;
STEERE, AC .
JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (02) :313-318
[10]   WESTERN BLOTTING IN THE SERODIAGNOSIS OF LYME-DISEASE [J].
DRESSLER, F ;
WHALEN, JA ;
REINHARDT, BN ;
STEERE, AC .
JOURNAL OF INFECTIOUS DISEASES, 1993, 167 (02) :392-400