SCML2 Establishes the Male Germline Epigenome through Regulation of Histone H2A Ubiquitination

被引：117

作者：

Hasegawa, Kazuteru ^{[1
,4
]}

Sin, Ho-Su ^{[1
,4
]}

Maezawa, So ^{[1
,4
]}

Broering, Tyler J. ^{[1
,4
]}

Kartashov, Andrey V. ^{[2
,4
]}

Alavattam, Kris G. ^{[1
,4
]}

Ichijima, Yosuke ^{[1
,4
]}

Zhang, Fan ^{[3
,4
]}

Bacon, W. Clark ^{[2
,4
]}

Greis, Kenneth D. ^{[5
]}

Andreassen, Paul R. ^{[3
,4
]}

Barski, Artem ^{[2
,4
]}

Namekawa, Satoshi H. ^{[1
,4
]}

机构：

[1] Cincinnati Childrens Hosp Med Ctr, Div Reprod Sci, Div Dev Biol, Perinatal Inst, Cincinnati, OH 45229 USA

[2] Cincinnati Childrens Hosp Med Ctr, Div Allergy & Immunol, Div Human Genet, Cincinnati, OH 45229 USA

[3] Cincinnati Childrens Hosp Med Ctr, Div Expt Hematol & Canc Biol, Cincinnati, OH 45229 USA

[4] Univ Cincinnati, Coll Med, Dept Pediat, Cincinnati, OH 45229 USA

[5] Univ Cincinnati, Coll Med, Dept Canc Biol, Cincinnati, OH 45229 USA

来源：

DEVELOPMENTAL CELL | 2015年 / 32卷 / 05期

基金：

日本学术振兴会;

关键词：

POSTMEIOTIC SEX-CHROMATIN; DNA-DAMAGE RESPONSE; MOUSE X-CHROMOSOME; STEM-CELLS; POLYCOMB COMPLEXES; GENE-EXPRESSION; DIFFERENTIATION; UBIQUITYLATION; TRANSCRIPTOME; TESTIS;

D O I：

10.1016/j.devcel.2015.01.014

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Gametogenesis is dependent on the expression of germline-specific genes. However, it remains unknown how the germline epigenome is distinctly established from that of somatic lineages. Here we show that genes commonly expressed in somatic lineages and spermatogenesis-progenitor cells undergo repression in a genome-wide manner in late stages of the male germline and identify underlying mechanisms. SCML2, a germline-specific subunit of a Polycomb repressive complex 1 (PRC1), establishes the unique epigenome of the male germline through two distinct antithetical mechanisms. SCML2 works with PRC1 and promotes RNF2-dependent ubiquitination of H2A, thereby marking somatic/progenitor genes on autosomes for repression. Paradoxically, SCML2 also prevents RNF2-dependent ubiquitination of H2A on sex chromosomes during meiosis, thereby enabling unique epigenetic programming of sex chromosomes for male reproduction. Our results reveal divergent mechanisms involving a shared regulator by which the male germline epigenome is distinguished from that of the soma and progenitor cells.

引用

页码：574 / 588

页数：15

共 65 条

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