Identification of quercetin glucuronides in human plasma by high-performance liquid chromatography-tandem mass spectrometry

被引：109

作者：

Wittig, J

Herderich, M

Graefe, EU

Veit, M

机构：

[1] Zent Inst Arzneimittelforsch, D-53789 Sinzig, Germany

[2] Univ Wurzburg, Inst Pharm & Food Chem, D-97074 Wurzburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 753卷 / 02期

关键词：

quercetin glucuronide; quercetin; MS/MS; SRM;

D O I：

10.1016/S0378-4347(00)00549-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

After intake of food or herbal medicinal products containing quercetin glycosides, the systemic availability of the genuine glycoside, as well as the systemic occurrence of the aglycone or conjugates of this polyphenol has been a matter of dispute. Consequently, we designed this study to develop a reliable method for determination of quercetin and its metabolites. Following consumption of fried onions five different glucuronides of quercetin could be identified in human plasma samples by means of HPLC-UV-MS/MS. Selective determination of the target compounds was achieved by simultaneous UV (254 nm) and MS/MS detection with selected reaction monitoring experiments using positive mode electrospray ionisation. In contrast, neither the free flavonol nor the genuine glycoside could be detected in plasma. Identification of the quercetin glucuronides detected in vivo was confirmed by comparison with authentic reference compounds synthesised enzymatically using glucuronyl transferase from rabbit liver. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：237 / 243

页数：7

共 14 条

[1] Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions [J].