Characterization and transcription of the genes encoding enzymes involved in butyrate production in Butyrivibrio fibrisolvens

被引:11
作者
Asanuma, N [1 ]
Kawato, M [1 ]
Ohkawara, S [1 ]
Hino, T [1 ]
机构
[1] Meiji Univ, Coll Agr, Dept Life Sci, Tama Ku, Kawasaki, Kanagawa 2148571, Japan
关键词
D O I
10.1007/s00284-002-3976-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Genes encoding enzymes that catalyze butyryl-CoA formation from acetyl-CoA in a type II strain of Butyrivibrio fibrisolvens were analyzed. The genes encoding thiolase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins were clustered, but the crotonase gene was not present in this region. The deduced amino acid sequences of these enzymes were similar to those of clostridia. The clustered genes were shown to be cotranscribed. The rate of butyrate production increased with an increase in acetate concentration in the medium up to 5 mm, suggesting that the butyryl-CoA/acetate CoA transferase reaction limits butyrate production. Transcription of the clustered genes was not affected by acetate concentration, suggesting that acetate does not affect the synthesis of enzymes involved in butyryl-CoA formation. These results confirm that acetate stimulates butyrate production by acting as a CoA acceptor in the butyryl-CoA/acetate CoA transferase reaction.
引用
收藏
页码:203 / 207
页数:5
相关论文
共 19 条
[1]   Structure and transcriptional regulation of the gene encoding pyruvate formate-lyase of a ruminal bacterium, Streptococcus bovis [J].
Asanuma, N ;
Iwamoto, M ;
Hino, T .
MICROBIOLOGY-UK, 1999, 145 :151-157
[2]   Presence of Butyrivibrio fibrisolvens in the digestive tract of dogs and cats, and its contribution to butyrate production [J].
Asanuma, N ;
Kawato, M ;
Hino, T .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 2001, 47 (06) :313-319
[3]   Regulation of lactate dehydrogenase synthesis in a ruminal bacterium, Streptococcus bovis [J].
Asanuma, N ;
Iwamoto, M ;
Hino, T .
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1997, 43 (06) :325-331
[4]  
Asanuma Narito, 1997, Animal Science and Technology, V68, P367
[5]   Phylogenetic relationships of butyrate-producing bacteria from the human gut [J].
Barcenilla, A ;
Pryde, SE ;
Martin, JC ;
Duncan, SH ;
Stewart, CS ;
Henderson, C ;
Flint, HJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (04) :1654-1661
[6]   Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824 [J].
Boynton, ZL ;
Bennett, GN ;
Rudolph, FB .
JOURNAL OF BACTERIOLOGY, 1996, 178 (11) :3015-3024
[7]   EFFECT OF PEPTIDES AND AMINO-ACIDS ON EFFICIENCY OF RUMEN BACTERIAL PROTEIN-SYNTHESIS IN CONTINUOUS CULTURE [J].
COTTA, MA ;
RUSSELL, JB .
JOURNAL OF DAIRY SCIENCE, 1982, 65 (02) :226-234
[8]   Alternative schemes of butyrate production in Butyrivibrio fibrisolvens and their relationship to acetate utilization, lactate production, and phylogeny [J].
Diez-Gonzalez, F ;
Bond, DR ;
Jennings, E ;
Russell, JB .
ARCHIVES OF MICROBIOLOGY, 1999, 171 (05) :324-330
[9]   16S rDNA analysis of Butyrivibrio fibrisolvens: Phylogenetic position and relation to butyrate-producing anaerobic bacteria from the rumen of white-tailed deer [J].
Forster, RJ ;
Teather, RM ;
Gong, J .
LETTERS IN APPLIED MICROBIOLOGY, 1996, 23 (04) :218-222
[10]   Butyrate acts as a survival factor for colonic epithelial cells: Further fuel for the in vivo versus in vitro debate [J].
Hague, A ;
Singh, B ;
Paraskeva, C .
GASTROENTEROLOGY, 1997, 112 (03) :1036-1040