Protective effect of prolactin and placental lactogen on NO-induced Nb2 lymphoma cell apoptosis

被引：8

作者：

Fernández, ML ^{[1
]}

Iglesias, MM ^{[1
]}

Biron, VA ^{[1
]}

Wolfenstein-Todel, C ^{[1
]}

机构：

[1] Univ Buenos Aires, Inst Quim & Fisicoquim Biol, CONICET, Fac Farm & Bioquim, RA-1113 Buenos Aires, DF, Argentina

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2003年 / 416卷 / 02期

关键词：

apoptosis; NO-donors; placental lactogen; prolactin; Nb2 lymphoma cells;

D O I：

10.1016/S0003-9861(03)00292-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide (NO) is an important modulator involved in immune regulation. Here, we describe conditions under which NO-donors induce apoptosis on Nb2 lymphoma cells, as evidenced by decreased cell viability and increased hypodiploid DNA content determined by flow cytometry. In addition, DNA fragmentation typical of apoptosis was shown by agarose gel electrophoresis. This apoptosis was accompanied by a significant increase of caspase-3-like enzymatic activity. Both ovine prolactin (oPRL) and ovine placental lactogen (oPL) exerted a protective effect on the NO-donor-induced apoptosis. Furthermore, dexamethasone (Dex)-induced cell death was also associated with caspase-3-like activity and oPL had the same potency as oPRL in its protective effect on Dex-induced apoptosis of Nb2 cells. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：249 / 256

页数：8

共 55 条

[1] Possible role for protein kinase B in the anti-apoptotic effect of prolactin in rat Nb2 lymphoma cells [J].