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Covalent immobilization of DNA on CVD diamond films

被引：18
作者：
Wenmackers, S
Haenen, K
Nesládek, M
Wagner, P
Michiels, L
vandeVen, M
Ameloot, M
机构：
[1] Limburgs Univ Centrum, Mat Res Inst, B-3590 Diepenbeek, Belgium
[2] Linburgs Univ Centrum, Biomed Res Inst, B-3590 Diepenbeek, Belgium
来源：
PHYSICA STATUS SOLIDI A-APPLIED RESEARCH | 2003年 / 199卷 / 01期
关键词：
D O I：
10.1002/pssa.200303822
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Chemical vapour deposited (CVD) diamond is used in DNA immobilization experiments for its high stability and versatility. In this study a double-stranded, 250 base pairs long DNA fragment of the human PKU gene was covalently bound to CVD diamond films. Thymidine was used as a linker molecule for the binding in head-on configuration. The presence of surface-bound FITC-labelled DNA was confirmed by confocal fluorescence microscopy. In denaturation experiments the second, unlabelled strand of each pair was removed resulting in a loss of fluorescence. The possibility of non-specific binding of DNA to diamond can be excluded. (C) 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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收藏
页码：44 / 48
页数：5
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