Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1

被引：42

作者：

Dobrosotskaya, IY ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Mol Genet, Grad Program Cell Regulat, Dallas, TX 75390 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 283卷 / 04期

关键词：

MAGI-1; NET1; MAGUK; cell junction; small G protein; Rho;

D O I：

10.1006/bbrc.2001.4880

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper reports the identification of a Rho family nucleotide exchange factor termed mNET1 as a candidate-interacting partner for the first PDZ domain of MAGI-1, a membrane-associated guanylate kinase with inverted arrangement of protein-protein interacting modules, mNET1 was identified in a yeast two-hybrid screen and has a consensus tripeptide for PDZ domain binding at its extreme carboxy-terminus. In addition to this sequence, a cluster of basic residues located near the carboxy terminus is essential for the binding. The interaction of the first PDZ domain of MAGI-1 with mNET1 was documented using a variety of biochemical methods. (C) 2001 Academic Press.

引用

页码：969 / 975

页数：7

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