The visual cycle retinol dehydrogenase:: possible involvement in the 9-cis retinoic acid biosynthetic pathway

被引:42
作者
Driessen, CAGG [1 ]
Winkens, HJ [1 ]
Kuhlmann, ED [1 ]
Janssen, APM [1 ]
van Vugt, AHM [1 ]
Deutmann, AF [1 ]
Janssen, JJM [1 ]
机构
[1] Univ Nijmegen, Dept Ophthalmol, NL-6525 EX Nijmegen, Netherlands
关键词
retinoid metabolism; retinol dehydrogenase; substrate specificity; visual cycle; retinoic acid;
D O I
10.1016/S0014-5793(98)00473-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The 11-cis-retinol dehydrogenase (11-cis-RoDH) gene encodes the short-chain alcohol dehydrogenase responsible for 11-cis-retinol oxidation in the visual cycle. The structure of the murine 11-cis-RoDH gene was used to reinvestigate its transcription pattern, An 11-cis-RoDH gene transcript nas detected in several non-ocular tissues, The question regarding the substrate specificity of the enzyme was therefore addressed. Recombinant 11-cis-RoDH was found capable of oxidizing and reducing 9-cis-, 11-cis- and 13-cis-isomers of retinol and retinaldehyde, respectively. Dodecyl-beta-1-maltoside used to solubilize the enzyme mas found to affect the substrate specificity. This is the first report on a visual cycle enzyme also present in non-retinal ocular and non-ocular tissues. A possible role in addition to its role in the visual cycle is being discussed. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:135 / 140
页数:6
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