Metabolism of the tomato saponin α-tomatine by Gibberella pulicaris

被引：14

作者：

Weltring, KM

Wessels, J

Pauli, GF

机构：

[1] Univ Munster, Inst Bot & Bot Garten, D-48149 Munster, Germany

[2] Univ Munster, Inst Pharmazeut Biol & Phytochem, D-48149 Munster, Germany

来源：

PHYTOCHEMISTRY | 1998年 / 48卷 / 08期

关键词：

Gibberella pulicaris; Fusarium; fungi; metabolism; saponins; steroid alkaloids; tomatine; tomato;

D O I：

10.1016/S0031-9422(97)00847-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ascomycete Gibberella pulicaris is able to metabolize the saponin alpha-tomatine within 2 h by first removing the complete lycotetraose moiety leading to the aglycone tomatidine. This product is further converted into 7 alpha-hydroxy-tomatidine and the corresponding Delta(5)-dehydro product 7 alpha-hydroxy-tomatidenol. Structural evidence for the hydroxylated main metabolite as well as the unsaturated minor product is based on MS and NMR measurements. The latter include the application of modern soft pulse H-1 NMR techniques allowing the definitive localization of the position of hydroxylation even for the few milligram quantities that could be obtained. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：1321 / 1328

页数：8

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[2]

ARNESON PA, 1968, PHYTOPATHOLOGY, V58, P536

[3] SURVEY OF FUSARIUM-SAMBUCINUM (GIBBERELLA-PULICARIS) FOR MATING TYPE, TRICHOTHECENE PRODUCTION, AND OTHER SELECTED TRAITS [J].