Kanyawara Virus: A Novel Rhabdovirus Infecting Newly Discovered Nycteribiid Bat Flies Infesting Previously Unknown Pteropodid Bats in Uganda

被引:39
作者
Goldberg, Tony L. [1 ,2 ,3 ]
Bennett, Andrew J. [1 ]
Kityo, Robert [3 ]
Kuhn, Jens H. [4 ]
Chapman, Colin A. [3 ,5 ,6 ]
机构
[1] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA
[2] Univ Wisconsin, Global Hlth Inst, Madison, WI 53706 USA
[3] Makerere Univ, Dept Zool, Kampala, Uganda
[4] NIAID, Integrated Res Facil Ft Detrick, NIH, Ft Detrick, MD 21702 USA
[5] McGill Univ, Dept Anthropol, Montreal, PQ H3A 2T7, Canada
[6] McGill Univ, Sch Environm, Montreal, PQ H3A 2T7, Canada
来源
SCIENTIFIC REPORTS | 2017年 / 7卷
基金
加拿大自然科学与工程研究理事会; 美国国家卫生研究院;
关键词
CODON USAGE; RNA; HIPPOBOSCOIDEA; TRANSMISSION; PHYLOGENIES; STREBLIDAE; DIVERSITY; EVOLUTION; ALIGNMENT; RESERVOIR;
D O I
10.1038/s41598-017-05236-w
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bats are natural reservoir hosts of highly virulent pathogens such as Marburg virus, Nipah virus, and SARS coronavirus. However, little is known about the role of bat ectoparasites in transmitting and maintaining such viruses. The intricate relationship between bats and their ectoparasites suggests that ectoparasites might serve as viral vectors, but evidence to date is scant. Bat flies, in particular, are highly specialized obligate hematophagous ectoparasites that incidentally bite humans. Using next-generation sequencing, we discovered a novel ledantevirus (mononegaviral family Rhabdoviridae, genus Ledantevirus) in nycteribiid bat flies infesting pteropodid bats in western Uganda. Mitochondrial DNA analyses revealed that both the bat flies and their bat hosts belong to putative new species. The coding-complete genome of the new virus, named Kanyawara virus (KYAV), is only distantly related to that of its closest known relative, Mount Elgon bat virus, and was found at high titers in bat flies but not in blood or on mucosal surfaces of host bats. Viral genome analysis indicates unusually low CpG dinucleotide depletion in KYAV compared to other ledanteviruses and rhabdovirus groups, with KYAV displaying values similar to rhabdoviruses of arthropods. Our findings highlight the possibility of a yet-to-be-discovered diversity of potentially pathogenic viruses in bat ectoparasites.
引用
收藏
页数:8
相关论文
共 61 条
[1]   TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations [J].
Abascal, Federico ;
Zardoya, Rafael ;
Telford, Maximilian J. .
NUCLEIC ACIDS RESEARCH, 2010, 38 :W7-W13
[2]   Adjusting for multiple testing when reporting research results: The Bonferroni vs Holm methods [J].
Aickin, M ;
Gensler, H .
AMERICAN JOURNAL OF PUBLIC HEALTH, 1996, 86 (05) :726-728
[3]   Taxonomy of the order Mononegavirales: update 2017 [J].
Amarasinghe, Gaya K. ;
Bao, Yiming ;
Basler, Christopher F. ;
Bavari, Sina ;
Beer, Martin ;
Bejerman, Nicolas ;
Blasdell, Kim R. ;
Bochnowski, Alisa ;
Briese, Thomas ;
Bukreyev, Alexander ;
Calisher, Charles H. ;
Chandran, Kartik ;
Collins, Peter L. ;
Dietzgen, Ralf G. ;
Dolnik, Olga ;
Duerrwald, Ralf ;
Dye, John M. ;
Easton, Andrew J. ;
Ebihara, Hideki ;
Fang, Qi ;
Formenty, Pierre ;
Fouchier, Ron A. M. ;
Ghedin, Elodie ;
Harding, Robert M. ;
Hewson, Roger ;
Higgins, Colleen M. ;
Hong, Jian ;
Horie, Masayuki ;
James, Anthony P. ;
JiAng, Daohong ;
Kobinger, Gary P. ;
Kondo, Hideki ;
Kurath, Gael ;
Lamb, Robert A. ;
Lee, Benhur ;
Leroy, Eric M. ;
Li, Ming ;
Maisner, Andrea ;
Muhlberger, Elke ;
Netesov, Sergey V. ;
Nowotny, Norbert ;
Patterson, Jean L. ;
Payne, Susan L. ;
Paweska, Janusz T. ;
Pearson, Michael N. ;
Randall, Rick E. ;
Revill, Peter A. ;
Rima, Bertus K. ;
Rota, Paul ;
Rubbenstroth, Dennis .
ARCHIVES OF VIROLOGY, 2017, 162 (08) :2493-2504
[4]  
[Anonymous], 2016, BIOSTRINGS STRING OB
[5]  
[Anonymous], 2019, R: A language for environment for statistical computing
[6]  
[Anonymous], METAGENOMIC ASSESSME
[7]  
[Anonymous], INT COMMITTEE TAXONO
[8]  
[Anonymous], 2011, Virus Taxonomy Ninth Report of the International Committee on Taxonomy of Viruses
[9]  
[Anonymous], ECTOPARASITES PANAMA
[10]   Detection of rhabdovirus viral RNA in oropharyngeal swabs and ectoparasites of Spanish bats [J].
Aznar-Lopez, Carolina ;
Vazquez-Moron, Sonia ;
Marston, Denise A. ;
Juste, Javier ;
Ibanez, Carlos ;
Miguel Berciano, Jose ;
Salsamendi, Egoitz ;
Aihartza, Joxerra ;
Banyard, Ashley C. ;
McElhinney, Lorraine ;
Fooks, Anthony R. ;
Echevarria, Juan .
JOURNAL OF GENERAL VIROLOGY, 2013, 94 :69-75