RNA-Directed Packaging of Enzymes within Virus-like Particles

被引：180

作者：

Fiedler, Jason D. ^{[1
,2
]}

Brown, Steven D. ^{[1
,2
]}

Lau, Jolene L. ^{[1
,2
]}

Finn, M. G. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 50期

基金：

美国国家卫生研究院;

关键词：

encapsulation; enzymes; Q beta bacteriophage; RNA-protein interactions; virus-like particles; CARRIER PROTEIN DOMAIN; BACTERIOPHAGE-Q-BETA; FIREFLY LUCIFERASE; VAULT NANOCAPSULES; DIFFUSION CONSTANT; COAT PROTEIN; IMMOBILIZATION; ENCAPSIDATION; DISPLAY; BINDING;

D O I：

10.1002/anie.201005243

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Packaged molecular machines are available using dual expression vectors that guide the preparation of Qβ virus-like particles encapsulating multiple enzymes. Packaging is promoted by RNA aptamer sequences that bridge between the coat protein and a peptide tag fused to the desired cargo (see scheme). Peptidase E and luciferase were thus encapsulated and shown to be catalytically active inside the particle. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9648 / 9651

页数：4

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