Enzymatic protein digest in chip-based nanovials with immobilized proteolytic enzymes

被引：12

作者：

Emmer, Å ^{[1
]}

Roeraade, J ^{[1
]}

机构：

[1] Royal Inst Technol, Dept Chem, Div Analyt Chem, SE-10044 Stockholm, Sweden

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 542卷 / 02期

关键词：

protein digest; immobilized enzymes; nanovials; capillary electrophoresis; MALDI-TOF MS;

D O I：

10.1016/j.aca.2005.03.062

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present work, protein digest reactions in silicon-based microchips, coated with immobilized proteolytic enzymes, have been carried out. The performance of such vials, modified with trypsin or chymotrypsin, was tested with myoglobin as a substrate. Capillary electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry were utilized for analysis of the digests, and the influence of different instrumentation setups. immobilization procedures and reaction conditions are discussed. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：137 / 143

页数：7

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