Phylogenetic relationships of Xylella fastidiosa strains from different hosts, based on 16S rDNA and 16S-23S intergenic spacer sequences

被引：52

作者：

Mehta, A ^{[1
]}

Rosato, YB ^{[1
]}

机构：

[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2001年 / 51卷

关键词：

Xylella fastidiosa; rDNA; DNA-DNA hybridization;

D O I：

10.1099/00207713-51-2-311

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The phylogenetic relationships of Xylella fastidiosa strains isolated from different hosts, including citrus trees, coffee, grapevine, plum and pear, were inferred by sequence analysis of the 16S rDNA and 16S-23S intergenic spacer region, A high level of similarity (97.1-100 %) was found in the 16S rDNA of the Xylella fastidiosa strains, The 16S-23S region showed a higher level of variation, with similarity values ranging from 79.8 to 100%. Two tRNAs (tRNA(Ala) and tRNA(Ile)) were encountered within the spacer sequence, The phylogenetic trees, constructed using the neighbour-joining method, showed that the citrus, coffee, peach and plum strains were closely related and separate from grapevine strains, The pear strain remained isolated from all the other Xylella strains in both analyses and produced values of less than 20% in DNA-DNA hybridization experiments with a citrus strain, These results show that this strain does not belong to the Xylella fastidiosa genomic species.

引用

页码：311 / 318

页数：8

共 39 条

[1] CULTURE AND SEROLOGICAL DETECTION OF THE XYLEM-LIMITED BACTERIUM CAUSING CITRUS VARIEGATED CHLOROSIS AND ITS IDENTIFICATION AS A STRAIN OF XYLELLA-FASTIDIOSA [J].

CHANG, CJ ;

GARNIER, M ;

ZREIK, L ;

ROSSETTI, V ;

BOVE, JM .

CURRENT MICROBIOLOGY, 1993, 27 (03) :137-142

[2] GENETIC-VARIATION AMONG XYLELLA-FASTIDIOSA STRAINS [J].

CHEN, J ;

CHANG, CJ ;

JARRET, RL ;

GAWEL, N .

PHYTOPATHOLOGY, 1992, 82 (09) :973-977

[3] Use of 16S rDNA sequences as signature characters to identify Xylella fastidiosa [J].

Chen, JC ;

Banks, D ;

Jarret, RL ;

Chang, CJ ;

Smith, BJ .

CURRENT MICROBIOLOGY, 2000, 40 (01) :29-33

[4]

CHEN T, 1995, BIOLINK, V4, P2

[5] ISOLATION MEDIA FOR THE PIERCES DISEASE BACTERIUM [J].

DAVIS, MJ ;

PURCELL, AH ;

THOMSON, SV .

PHYTOPATHOLOGY, 1980, 70 (05) :425-429

[6]

DAVIS MJ, 1980, PROKARYOTES HDB HABI, P2172

[7] GENOTYPIC RELATIONSHIPS AND TAXONOMIC LOCALIZATION OF UNCLASSIFIED PSEUDOMONAS AND PSEUDOMONAS-LIKE STRAINS BY DEOXYRIBONUCLEIC-ACID - RIBOSOMAL RIBONUCLEIC-ACID HYBRIDIZATIONS [J].

DEVOS, P ;

VANLANDSCHOOT, A ;

SEGERS, P ;

TYTGAT, R ;

GILLIS, M ;

BAUWENS, M ;

ROSSAU, R ;

GOOR, M ;

POT, B ;

KERSTERS, K ;

LIZZARAGA, P ;

DELEY, J .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (01) :35-49

[8]

FILHO OP, 1997, SUMMA PHYTOPATHOL, V23, P46

[9]

FRENCH WJ, 1978, PLANT DIS REP, V62, P1035

[10] Genotypic characterization of xanthomonad strains isolated from passion fruit plants (Passiflora spp.) and their relatedness to different Xanthomonas species [J].

Gonçalves, ER ;

Rosato, YB .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :811-821

← 1 2 3 4 →