Liquid chromatography-tandem mass spectrometry assays for intracellular deoxyribonucleotide triphosphate competitors of nucleoside antiretrovirals

被引：53

作者：

Henneré, G

Becher, F

Pruvost, A

Goujard, C

Grassi, J

Benech, H ^{[1
]}

机构：

[1] CEA Saclay, DRM, DSV, Serv Pharmacol & Immunol, F-91191 Gif Sur Yvette, France

[2] Hop Univ Bicetre, Serv Med Interne, Le Kremlin Bicetre, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 789卷 / 02期

关键词：

deoxyribonucleotide triphosphate; nucleosides;

D O I：

10.1016/S1570-0232(03)00099-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was aimed to apply an LC-MS-MS method previously developed for intracellular nucleoside reverse transcriptase inhibitors-triphosphate (NRTI-TPs) to the determination of natural deoxyribonucleotides (dNTPs) in human peripheral blood mononuclear cells. The LC-MS-MS method was directly used in assay of dATP and dTTP. Interferences by ribonucleotides (rNTPs) prevented direct application to the two other analytes: dGTP and dCTR A periodate oxidation procedure was therefore optimized to remove rNTPs from the cell medium in order to quantitate dCTP and dGTP. The determination of the intracellular ratio of NRTI-TP/dNTP in HIV-infected patients now involves use of the same chromatographic system for simultaneous assay of several analytes. (C) 2003 Elsevier Science B.V. All rights reserved.

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页码：273 / 281

页数：9

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