Genetic mapping in pea.: 1.: RAPD-based genetic linkage map of Pisum sativum

被引:92
作者
Laucou, V
Haurogné, K
Ellis, N
Rameau, C
机构
[1] INRA, Genet & Ameliorat Plantes Stn, F-78026 Versailles, France
[2] John Innes Ctr Plant Sci Res, Dept Appl Genet, Norwich NR4 7UH, Norfolk, England
关键词
linkage mapping; Pisum sativum; random amplified polymorphic DNA; recombinant inbred lines;
D O I
10.1007/s001220050971
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A genetic linkage map of Pisum sativum L. was constructed,based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich dry-seed cultivar Terese and an increased branching mutant (K586) obtained from the pea cultivar 'Torsdag', The map currently comprises nine linkage groups with two groups comprising only 6 markers (n = 7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281 x JI399) RILs population that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed. For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.
引用
收藏
页码:905 / 915
页数:11
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