A novel biosensor system for the determination of phosphate

被引：44

作者：

Ikebukuro, K

Nishida, R

Yamamoto, H

Arikawa, Y

Nakamura, H

Suzuki, M

Kubo, I

Takeuchi, T

Karube, I

机构：

[1] UNIV TOKYO,ADV SCI & TECHNOL RES CTR,MEGURO KU,TOKYO 153,JAPAN

[2] JAPAN WOMENS UNIV,FAC SCI,DEPT CHEM & BIOL SCI,BUNKYO KU,TOKYO 112,JAPAN

[3] TSURUMI SEIKI CO LTD,TSURUMI KU,YOKOHAMA,KANAGAWA,JAPAN

[4] KYUSHU INST TECHNOL,FAC COMP SCI & SYST ENGN,DEPT BIOCHEM ENGN SCI,IIZUKA,FUKUOKA 820,JAPAN

[5] SOKA UNIV,FAC ENGN,DEPT BIOENGN,HACHIOJI,TOKYO 192,JAPAN

[6] HIROSHIMA CITY UNIV,FAC INFORMAT SCI,ASANAMI KU,HIROSHIMA 73113,JAPAN

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 48卷 / 1-2期

关键词：

phosphate; luminol; chemiluminescence; pyruvate oxidase; flow injection analysis;

D O I：

10.1016/0168-1656(96)01398-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A highly sensitive biosensor system has been developed for phosphate detection. This biosensor system is based on the pyruvate oxidase reaction and a subsequent luminol chemiluminescence reaction. Hydrogen peroxide generated by pyruvate oxidase reacts with luminol catalyzed by an immobilized peroxidase. The resulting chemiluminescence is detected by a photomultiplier. This system uses a flow injection analysis (FIA) system resulting in the rapid determination of phosphate, taking approximately 3 min for one measurement. A linear response was observed from 0.37 mu M to 7.4 mu M phosphate while the detection limit was 74 nM. This sensitivity is sufficient to determine the maximum permissible phosphate concentration of the natural waters of Japan (0.32 mu M). The pyruvate oxidase immobilized column gave a stable response over a period of 2 weeks when stock solutions were analyzed.

引用

页码：67 / 72

页数：6

共 15 条

[1] CHEMILUMINESCENT ANALYTE MICRODETECTION BASED ON THE LUMINOL-H2O2 REACTION USING PEROXIDASE IMMOBILIZED ON NEW SYNTHETIC MEMBRANES [J].