Efficacy of photocrosslinkable chitosan hydrogel containing fibroblast growth factor-2 in a rabbit model of chronic myocardial infarction

被引:73
作者
Fujita, M
Ishihara, M
Morimoto, Y
Simizu, M
Saito, Y
Yura, H
Matsui, T
Takase, B
Hattori, H
Kanatani, Y
Kikuchi, M
Maehara, T
机构
[1] Natl Def Med Coll, Dept Med Engn, Tokorozawa, Saitama 3598513, Japan
[2] Natl Def Med Coll, Dept Surg 2, Tokorozawa, Saitama 3598513, Japan
[3] Natl Def Med Coll, Res Inst, Tokorozawa, Saitama 3598513, Japan
[4] NeTech Inc, Kanagawa, Japan
关键词
photocrosslinkable chitosan hydrogel; fibroblast growth factor-2; myocardial infaretion; coronary artery; vascularization; drug delivery system;
D O I
10.1016/j.jss.2004.12.025
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. Therapeutic angiogenesis in ischemic myocardium. has been shown to be an effective strategy to improve regional blood flow and myocardial function. However, no effective delivery system for growth factor administration is yet known to induce important therapeutic angiogenic responses in ischemic myocardium. Materials and methods. FGF-2-incorporated chitosan (FGF-2/chitosan) hydrogels were immobilized on the surface of ischemic myocardium of rabbit models of chronic myocardial infarction by UV-irradiation. After 4 weeks, cardiac functional analyses by noradrenalin challenge and histopathological analyses were performed to evaluate the efficacy of a controlled release of FGF-2 from FGF-2/chitosan hydrogel immobilized on the surface of ischemic myocardium. Results. Significant improvement by application of FGF-2/chitosan hydrogels was found in systolic pressure at the left ventricle, +dp/dt maximum, and -dp/dt maximum during noradrenalin challenge at a dose of 1 mu g/kg/min. Histological observations showed that a significantly larger amount of viable myocardium and CD 31 immunostained blood vessels were found in the FGF-2/chitosan hydrogel-applied group than only the chitosan-applied and control groups. Conclusions. These preliminary results indicate that the controlled release of biologically active FGF-2 molecules from FGF-2/chitosan hydrogel induces angiogenesis and possibly collateral circulation in ischemic myocardium, thereby protecting the myocardium. (C) 2005 Elsevier Inc. All rights reserved.
引用
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页码:27 / 33
页数:7
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