Isolation and analysis of cinnamic acid 4-hydroxylase homologous genes from a hybrid aspen, Populus kitatamiensis

被引：35

作者：

Kawai, S ^{[1
]}

Mori, A ^{[1
]}

Shiokawa, T ^{[1
]}

Kajita, S ^{[1
]}

Katayama, Y ^{[1
]}

Morohoshi, N ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL, GRAD SCH BIOAPPLICAT & SYST ENGN, FUCHU, TOKYO 183, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 10期

关键词：

CYP73; cytochrome P-450; cinnamic acid 4-hydroxylase; phenylpropanoid; Populus kitakamiensis;

D O I：

10.1271/bbb.60.1586

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cinnamic acid 4-hydroxylase (CA4H) is the second enzyme involved in phenylpropanoid biosynthesis and is a member of the cytochrome P-450 superfamily. Three CA4H homologous genes, cyp73a, cyp73b, and cyp73c, and a cDNA clone of cyp73a were isolated from a genomic library and a cDNA library of a hybrid aspen; Populus ktakamiensis, and were characterized. They might be interrupted to two introns each. cyp73a and cyp73b were very similar to each other not only in coding regions but also in non-coding regions. Southern blot analysis showed that four homologous genes for CA4H constructed a small gene family in the diploid genome of P. kitakamienis. In the promoter regions, there were many common cis-element-like sequences in phenylpropanoid biosynthesis genes.

引用

页码：1586 / 1597

页数：12

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