Increase of galanin mRNA in lumbar dorsal root ganglion neurons of adult rats after partial sciatic nerve ligation

被引：14

作者：

Ma, WY ^{[1
]}

Bisby, MA ^{[1
]}

机构：

[1] Queens Univ, Fac Med, Dept Physiol, Kingston, ON K7L 3N6, Canada

来源：

NEUROSCIENCE LETTERS | 1999年 / 262卷 / 03期

基金：

英国医学研究理事会;

关键词：

in situ hybridization; fluorescent dye tracing; sensory; nerve injury; galanin; neuropathic pain; primary sensory neuron;

D O I：

10.1016/S0304-3940(99)00085-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Partial sciatic nerve ligation (PSNL) is a widely used model for the study of neuropathic pain. However, there is little information on neuropeptide expression in primary sensory neurons after PSNL. We examined galanin (GAL) mRNA expression in L4 and L5 dorsal root ganglion (DRG) neurons of adult rats after PSNL. We found that 4 and 14 days after PSNL the percentages of GAL mRNA positive neurons were significantly increased in the ipsilateral DRG compared to the contralateral side. Using combined retrograde fluorescent dye tracing and in situ hybridization, we found that 47% of the injured neurons and 10% of the spared neurons were GAL mRNA positive. Since only 2-3% of neurons in the contralateral uninjured DRG were GAL rnRNA positive, PSNL induced up-regulation of GAL mRNA in both injured and spared DRG neurons. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：195 / 198

页数：4

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