Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells

被引:356
作者
Park, Tae Joo [1 ,2 ]
Mitchell, Brian J. [3 ]
Abitua, Philip B. [1 ,2 ]
Kintner, Chris [3 ]
Wallingford, John B. [1 ,2 ]
机构
[1] Univ Texas, Dept Mol Cell & Dev Biol, Austin, TX 78712 USA
[2] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78712 USA
[3] Salk Inst Biol Studies, La Jolla, CA 92186 USA
关键词
D O I
10.1038/ng.104
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The planar cell polarity (PCP) signaling system governs many aspects of polarized cell behavior. Here, we use an in vivo model of vertebrate mucociliary epithelial development to show that Dishevelled (Dvl) is essential for the apical positioning of basal bodies. We find that Dvl and Inturned mediate the activation of the Rho GTPase specifically at basal bodies, and that these three proteins together mediate the docking of basal bodies to the apical plasma membrane. Moreover, we find that this docking involves a Dvl-dependent association of basal bodies with membrane-bound vesicles and the vesicle-trafficking protein, Sec8. Once docked, basal bodies again require Dvl and Rho for the planar polarization that underlies directional beating of cilia. These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies.
引用
收藏
页码:871 / 879
页数:9
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