Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy

被引：74

作者：

Kambhampati, D

Nielsen, PE

Knoll, W

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[2] Panum Inst, Ctr Biomol Recognit, Dept Med Biochem & Genet, DK-2200 Copenhagen, Denmark

来源：

BIOSENSORS & BIOELECTRONICS | 2001年 / 16卷 / 9-12期

关键词：

peptide nucleic acid (PNA); DNA; plasmon;

D O I：

10.1016/S0956-5663(01)00239-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Plasmon surface polaritons, resonantly excited in the Kretschmann format, are used to enhance the fluorescence emission of chromophore-labeled oligonucleotides (15mers) binding to surface-attached (via biotin-streptavidin linkages) complement catcher probes. A detailed analysis of the association and dissociation kinetics as well as the affinity constants is given for a mismatch I hybrid, emphasizing, in particular, the experimental conditions that are required to allow for an artifact-free determination of rate constants. A first comparison between DNA- and peptide nucleic acid (PNA-) probes shows similar affinities, however, significant deviations from single-exponential kinetics predicted by a simple Langmuir model for the PNA case are found. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：1109 / 1118

页数：10

共 14 条

[1] A multistep chemical modification procedure to create DNA arrays on gold surfaces for the study of protein-DNA interactions with surface plasmon resonance imaging
Brockman, JM
Frutos, AG
Corn, RM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (35) : 8044 - 8051
[2] MULTIPLEXED BIOCHEMICAL ASSAYS WITH BIOLOGICAL CHIPS
FODOR, SPA
RAVA, RP
HUANG, XHC
PEASE, AC
HOLMES, CP
ADAMS, CL
[J]. NATURE, 1993, 364 (6437) : 555 - 556
[3] Gotoh M, 1995, DNA Res, V2, P285, DOI 10.1093/dnares/2.6.285
[4] Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique
Jensen, KK
Orum, H
Nielsen, PE
Norden, B
[J]. BIOCHEMISTRY, 1997, 36 (16) : 5072 - 5077
[5] Single-base mismatch detection based on charge transduction through DNA
Kelley, SO
Boon, EM
Barton, JK
Jackson, NM
Hill, MG
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (24) : 4830 - 4837
[6] Streptavidin arrays as supramolecular architectures in surface-plasmon optical sensor formats
Knoll, W
Zizlsperger, M
Liebermann, T
Arnold, S
Badia, A
Liley, M
Piscevic, D
Schmitt, FJ
Spinke, J
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2000, 161 (01) : 115 - 137
[7] Interfaces and thin films as seen by bound electromagnetic waves
Knoll, W
[J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1998, 49 : 569 - 638
[8] Surface-plasmon field-enhanced fluorescence spectroscopy
Liebermann, T
Knoll, W
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2000, 171 (1-3) : 115 - 130
[9] Complement hybridization from solution to surface-attached probe-oligonucleotides observed by surface-plasmon-field-eahanced fluorescence spectroscopy
Liebermann, T
Knoll, W
Sluka, P
Herrmann, R
[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2000, 169 (1-3) : 337 - 350
[10] Kinetic measurements of DNA hybridisation an an oligonucleotide-immobilized 27-MHz quartz crystal microbalance
Okahata, Y
Kawase, M
Niikura, K
Ohtake, F
Furusawa, H
Ebara, Y
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (07) : 1288 - 1296

← 1 2 →